含有HIV前病毒DNA的转基因小鼠的疾病发展和病毒恢复情况
Development of disease and virus recovery in transgenic mice containing HIV proviral DNA.
作者信息
Leonard J M, Abramczuk J W, Pezen D S, Rutledge R, Belcher J H, Hakim F, Shearer G, Lamperth L, Travis W, Fredrickson T
机构信息
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
出版信息
Science. 1988 Dec 23;242(4886):1665-70. doi: 10.1126/science.3201255.
Transgenic mice containing intact copies of the human immunodeficiency virus (HIV) proviral DNA were constructed. Founder animals were not viremic for HIV and remained healthy during a 9-month observation period. After being mated with nontransgenic animals, one founder mouse (No. 13) gave rise to F1 progeny that developed a disease syndrome characterized by marked epidermal hyperplasia, lymphadenopathy, splenomegaly, pulmonary lymphoid infiltrates, growth retardation, and death by day 25 of life. Infectious HIV, indistinguishable from parental virus by immunoblot analysis, was recovered from the spleen, lymph nodes, and skin of five of five affected animals.
构建了含有完整人类免疫缺陷病毒（HIV）前病毒DNA拷贝的转基因小鼠。奠基动物未出现HIV病毒血症，在9个月的观察期内保持健康。与非转基因动物交配后，一只奠基小鼠（第13号）产生了F1代后代，这些后代在出生后第25天出现了以明显的表皮增生、淋巴结病、脾肿大、肺部淋巴浸润、生长发育迟缓以及死亡为特征的疾病综合征。通过免疫印迹分析，从五只受影响动物中的五只的脾脏、淋巴结和皮肤中分离出了与亲代病毒无法区分的传染性HIV。
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