血液曲霉聚合酶链反应：优点、缺点与问题
Blood Aspergillus PCR: The Good, the Bad, and the Ugly.
作者信息
Egger Matthias, Jenks Jeffrey D, Hoenigl Martin, Prattes Juergen
机构信息
Section of Infectious Diseases and Tropical Medicine, Medical University of Graz, 8036 Graz, Austria.
Department of Medicine, University of California San Diego, San Diego, 92093 CA, USA.
出版信息
J Fungi (Basel). 2020 Jan 27;6(1):18. doi: 10.3390/jof6010018.
Abstract
Invasive (IA) is one of the most common invasive fungal diseases and is accompanied by high morbidity and mortality. In order to maximize patient outcomes and survival, early and rapid diagnosis has been shown to be pivotal. Hence, diagnostic tools aiding and improving the diagnostic process are ambitiously searched for. In this context, polymerase chain reaction (PCR) may represent a potential candidate. Its additional value and benefits in diagnosis have been demonstrated and are scientifically established. Nevertheless, standardized and widespread usage is sparse because several factors influence diagnostic quality and need to be considered in order to optimize diagnostic performance and outcome. In the following review, the current role of PCR in the diagnosis of IA is explored, with special focus on the strengths and limitations of PCR in different settings.
摘要
侵袭性曲霉病（IA）是最常见的侵袭性真菌病之一，其发病率和死亡率都很高。为了使患者的治疗效果和生存率最大化，早期快速诊断已被证明至关重要。因此，人们一直在积极寻找有助于并改善诊断过程的诊断工具。在这种背景下，聚合酶链反应（PCR）可能是一个潜在的候选方法。其在诊断中的附加价值和益处已得到证实且有科学依据。然而，由于有几个因素会影响诊断质量，为了优化诊断性能和结果需要加以考虑，所以PCR的标准化和广泛应用并不多见。在以下综述中，我们探讨了PCR在IA诊断中的当前作用，特别关注其在不同情况下的优势和局限性。
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