Faculty of Engineering, Department of Environmental Engineering, Nigde Ömer Halisdemir University, Nigde, Turkey.
Faculty of Engineering, Department of Environmental Engineering, Nigde Ömer Halisdemir University, Nigde, Turkey.
Bioresour Technol. 2020 Aug;309:123365. doi: 10.1016/j.biortech.2020.123365. Epub 2020 Apr 10.
The effects of graphite on the anaerobic digestion of food waste (FW), cow manure (CM) and its mixture (FW/CM) via batch experiments under mesophilic conditions have been investigated in this study. Maximum biogas production with graphite addition for FW + 1 g/L, CM + 1.5 g/L and FW/CM + 0.75 g/L are 1128.46, 829.6 and 1471.1 mL/gVS respectively. Additionally, this study investigates the link between microbial community structure and biogas production when graphite addition of anaerobic digester was conducted. Based on 16S rRNA gene amplicon sequencing results, Aminiphilus (13-14%), Actinobaculum (13-15%) and Clostridium (12-18%) were the predominant bacterial genera in graphite-added FW, CM and FW/CM reactors, respectively. Comparatively higher biogas production of FW/CM synergistically affected by abundances of Clostridium as well as co-digestion in this anaerobic digestion setup. Methanosaeta was the most abundant methanogen in the graphite added digesters; however, the relative abundance of these genera was different.
本研究通过中温批式实验，考察了石墨对食物垃圾（FW）、牛粪（CM）及其混合物（FW/CM）厌氧消化的影响。添加石墨后，FW+1g/L、CM+1.5g/L 和 FW/CM+0.75g/L 的最大沼气产量分别为 1128.46、829.6 和 1471.1 mL/gVS。此外，本研究还研究了在添加石墨的厌氧消化器中，微生物群落结构与沼气产量之间的关系。基于 16S rRNA 基因扩增子测序结果，添加石墨后的 FW、CM 和 FW/CM 反应器中优势细菌属分别为 Aminiphilus（13-14%）、Actinobaculum（13-15%）和 Clostridium（12-18%）。与单独消化相比，FW/CM 协同作用下的较高沼气产量受到 Clostridium 丰度以及共消化的影响。在添加石墨的消化器中，Methanosaeta 是最丰富的产甲烷菌；然而，这些菌属的相对丰度不同。
