Walker K Z, Khafagi F, Bautovich G J, Boniface G R, Bundesen P G, Rylatt D B
Clinical Immunology Research Centre, Sydney University, N.S.W., Australia.
Thromb Res. 1988 Nov 15;52(4):269-78. doi: 10.1016/0049-3848(88)90068-0.
The D Dimer (DD) site formed by linkage of D domains from adjacent fibrin (FN) molecules is unique to cross-linked FN and its degradation products and is not found in FN monomer or fibrinogen (FB). Thus monoclonal antibodies (MAb) reactive to DD should have a very suitable specificity for in vivo thrombus detection. Two anti-DD MAbs have been labelled with 131-I and assessed as scintigraphic agents in a normal rat model system. Each rat received 3 sc implants of antigen covalently coupled to Sepharose beads: 1) Human DD 2) Human FB 3) Glycine (GL) (control). Scintigraphic images taken 7 days after injection of 131-I anti DD MAb showed clear localisation of both anti-DD MAbs to the DD implant rather than to the FB or GL implants with no localisation in normal tissues. This was confirmed in biodistribution studies. Injection of anti-DD MAbs DD-3B6/22 and DD-IC3/108 resulted in DD: blood ratios of 10.4 +/- 0.6 and 4.9 +/- 0.3 respectively. These results suggest that anti-DD MAbs will have potential for thrombus radioimmunodetection.
由相邻纤维蛋白（FN）分子的D结构域连接形成的D-二聚体（DD）位点是交联FN及其降解产物所特有的，在FN单体或纤维蛋白原（FB）中不存在。因此，对DD有反应性的单克隆抗体（MAb）对体内血栓检测应具有非常合适的特异性。两种抗DD MAb已用131-I标记，并在正常大鼠模型系统中作为闪烁显像剂进行评估。每只大鼠接受3次皮下植入与琼脂糖珠共价偶联的抗原：1）人DD 2）人FB 3）甘氨酸（GL）（对照）。注射131-I抗DD MAb 7天后拍摄的闪烁显像图像显示，两种抗DD MAb均清晰定位于DD植入物，而非FB或GL植入物，且在正常组织中无定位。这在生物分布研究中得到了证实。注射抗DD MAb DD-3B6/22和DD-IC3/108后，DD与血液的比率分别为10.4±0.6和4.9±0.3。这些结果表明，抗DD MAb在血栓放射免疫检测方面具有潜力。
