抗D二聚体单克隆抗体：一种用于血栓免疫检测的潜在闪烁显像剂。

Anti D dimer monoclonal antibodies: a possible scintigraphic agent for immunodetection of thrombi.

作者信息

Scopinaro F, Di Loreto M, Banci M, Schillaci O, Di Macio L, de Santis R, Anastasi A M, Ciancarella W, Centi Colella A, Mele A

机构信息

Department of Experimental Medicine, University La Sapienza, Rome, Italy.

出版信息

Nucl Med Commun. 1992 Oct;13(10):723-9.

PMID:1491836

Abstract

The aim of this work was to produce a MoAb able to react with clots but not with fibrinogen. Monoclonal antibodies directed towards DD dimers, against specific products of plasmic digestion of cross-linked fibrin, were obtained. One of these antibodies, F 60/43/8, showed a 1.79 x 10(9) l mol-1 binding constant in spite of the presence of fibrinogen at a 4000 times greater concentration than the cross-linked fibrin. In vitro studies with 125I-F(ab')2 of F 60/43/8 showed that 34-80% of the radioactivity can be found in human clots, in the presence of physiologic concentrations of fibrinogen, and that 96-h washing does not remove the labelled F(ab')2 from the clot. 131I-F(ab')2 was injected into rabbits in which a clot had formed in an artery (six rabbits) or in a vein (six rabbits) of the left ear. Scintigraphic images of the clot were always obtained. In conclusion, the results of this work suggest that F 60/43/8 may be used as a specific antibody for the radioimmunodetection of thrombi.

摘要

这项工作的目的是制备一种能与血凝块反应但不与纤维蛋白原反应的单克隆抗体（MoAb）。获得了针对交联纤维蛋白的血浆消化特异性产物——D - 二聚体的单克隆抗体。其中一种抗体F 60/43/8，尽管纤维蛋白原的浓度比交联纤维蛋白高4000倍，但其结合常数仍为1.79×10⁹ l·mol⁻¹。用F 60/43/8的¹²⁵I - F(ab')₂进行的体外研究表明，在生理浓度的纤维蛋白原存在下，34% - 80%的放射性可在人血凝块中检测到，并且96小时的洗涤也不能从血凝块中去除标记的F(ab')₂。将¹³¹I - F(ab')₂注入左耳动脉（6只兔子）或静脉（6只兔子）中已形成血凝块的兔子体内。总能获得血凝块的闪烁图像。总之，这项工作的结果表明F 60/43/8可作为血栓放射免疫检测的特异性抗体。