Lee F T, Milner L J, Boniface G R, Bautovich G J, Weedon A R, Bundesen P G, Rylatt D B, Walker K Z
ANSTO, Sydney, New South Wales, Australia.
Immunol Cell Biol. 1992 Jun;70 ( Pt 3):173-9. doi: 10.1038/icb.1992.22.
Technetium-99 m (99mTc)-labelled conjugates of an anti-fibrin monoclonal antibody, DD-3B6/22, have been assessed for their detection of vascular thrombi in a rabbit model. DD-3B6/22 binds to a D-dimer epitope present on cross-linked fibrin but absent from the fibrin monomer or fibrinogen. Injection of a 99mTc-labelled Fab' fragment of DD-3B6/22 allowed delineation of model thrombi as early as 30 min postinjection (p.i.) with optimal localization at 4-5 h. Thrombus label uptake at 4 h p.i. was 0.304 +/- 0.106% injected dose/g (% ID/g) compared with 0.022 +/- 0.001% ID/g after the injection of a control Fab' fragment. These results suggest that the 99mTc-labelled Fab' fragment of DD-3B6/22 has excellent potential for scintigraphic detection of vascular thrombi in humans.
已对一种抗纤维蛋白单克隆抗体DD-3B6/22的锝-99m（99mTc）标记结合物在兔模型中检测血管血栓的能力进行了评估。DD-3B6/22与交联纤维蛋白上存在但纤维蛋白单体或纤维蛋白原中不存在的D-二聚体表位结合。注射99mTc标记的DD-3B6/22 Fab′片段后，最早在注射后30分钟即可描绘出模型血栓，在4至5小时时定位最佳。注射后4小时血栓标记摄取量为0.304±0.106%注射剂量/克（%ID/g），而注射对照Fab′片段后为0.022±0.001%ID/g。这些结果表明，99mTc标记的DD-3B6/22 Fab′片段在人体血管血栓闪烁显像检测方面具有出色的潜力。
