Kinaci Ahmet, Bergmann Wilhelmina, Bleys Ronald Law, van der Zwan Albert, van Doormaal Tristan Pc
Department of Neurology and Neurosurgery, Brain Center Rudolph Magnus, University Medical Center Utrecht, Utrecht, The Netherlands; Brain Technology Institute, Utrecht, The Netherlands;, Email:
Division ofPathology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Comp Med. 2020 Apr 1;70(2):170-175. doi: 10.30802/AALAS-CM-19-000022. Epub 2020 Feb 3.
The biocompatibility, biodegradation, feasibility, and efficacy of medical devices like dural sealants and substitutes are often evaluated in various animal models. However, none of these studies explain the rationale for choosing a particular species, and a systematic interspecies comparison of the dura is not available. We hypothesized that histologic characteristics of the dura would differ among species. We systematically investigated basic characteristics of the dura, including thickness, composition, and fibroblast orientation of the dura mater, in 34 samples representing 10 animal species and compared these features with human dura by using hematoxylin and eosin staining and light microscopy. Dura showed many similarities between species in terms of composition. In all species, dura consisted of at least one fibrovascular layer, which contained collagen, fibroblasts, and blood vessels, and a dural border cell layer beneath the fibrovascular layer. Differences between species included the number of fibrovascular layers, fibroblast orientation, and dural thickness. Human dura was the thickest (564 μm) followed by equine (313 μm), bovine (311 μm), and porcine (304 μm) dura. Given the results of this study and factors such as gross anatomy, feasibility, housing, and ethical considerations, we recommend the use of a porcine model for dural research, especially for in vivo studies.
诸如硬脑膜封闭剂和替代物等医疗器械的生物相容性、生物降解性、可行性和有效性通常在各种动物模型中进行评估。然而，这些研究均未解释选择特定物种的理由，且缺乏对不同物种硬脑膜的系统比较。我们假设不同物种的硬脑膜组织学特征会有所不同。我们系统地研究了代表10种动物的34个样本中硬脑膜的基本特征，包括硬脑膜的厚度、组成和成纤维细胞方向，并通过苏木精-伊红染色和光学显微镜将这些特征与人类硬脑膜进行比较。硬脑膜在组成方面在不同物种之间表现出许多相似之处。在所有物种中，硬脑膜至少由一层纤维血管层组成，该层包含胶原蛋白、成纤维细胞和血管，以及纤维血管层下方的硬脑膜边界细胞层。不同物种之间的差异包括纤维血管层的数量、成纤维细胞方向和硬脑膜厚度。人类硬脑膜最厚（564μm），其次是马（313μm）、牛（311μm）和猪（304μm）的硬脑膜。鉴于本研究的结果以及大体解剖、可行性、饲养和伦理考量等因素，我们建议使用猪模型进行硬脑膜研究，尤其是体内研究。
