冷等离体子体对人骨肉瘤细胞膜通透性的影响。
The Effect of Cold Atmospheric Plasma on the Membrane Permeability of Human Osteosarcoma Cells.
机构信息
Department of Trauma, Reconstructive Surgery and Rehabilitation Medicine, University Medicine Greifswald, Greifswald, Germany
Department of Trauma and Orthopaedic Surgery, BG Klinikum Unfallkrankenhaus Berlin gGmbH, Berlin, Germany.
出版信息
Anticancer Res. 2020 Feb;40(2):841-846. doi: 10.21873/anticanres.14016.
BACKGROUND
Cold atmospheric plasma (CAP) has a variety of anticancer effects on different cancer cell types. In osteosarcoma (OS) cells, CAP reduces growth and motility, induces apoptosis, and alters secretion of cellular factors. The influence of CAP on membrane integrity of OS cells is unknown.
MATERIALS AND METHODS
Two different OS cell lines (U-2 OS and MNNG-HOS) were treated with CAP. Proliferation assays for cell growth after treatment was performed. Alterations in membrane permeability and the associated translocation of low molecular weight particles through the cytoplasmic membrane of OS cells after CAP treatment were shown in fluorescein diacetate (FDA) assays.
RESULTS
FDA increasingly passed the membrane after CAP treatment and this effect depended on the duration of treatment. It was also shown that after CAP treatment, FDA was able to diffuse into the cells from the outside as well as out of the cell interior. These effects were observed when CAP-treated buffer was used and therefore no direct contact between cells and CAP occurred.
CONCLUSION
The observations suggest that changes in membrane permeability and function may contribute to the antiproliferative effects of CAP.
背景
冷等离体气体（CAP）对不同类型的癌细胞具有多种抗癌作用。在骨肉瘤（OS）细胞中，CAP 可降低细胞生长和运动能力，诱导细胞凋亡，并改变细胞因子的分泌。CAP 对 OS 细胞膜完整性的影响尚不清楚。
材料和方法
用 CAP 处理两种不同的骨肉瘤细胞系（U-2 OS 和 MNNG-HOS）。用 CAP 处理后进行细胞生长增殖测定。通过荧光二乙酸（FDA）试验显示 CAP 处理后 OS 细胞的膜通透性改变及相关的低分子量颗粒通过细胞质膜的易位。
结果
CAP 处理后 FDA 越来越多地通过细胞膜，这种效应取决于处理时间的长短。还表明，CAP 处理后，FDA 能够从细胞外扩散到细胞内，也能从细胞内扩散到细胞外。当使用 CAP 处理的缓冲液时观察到这些效应，因此细胞与 CAP 之间没有直接接触。
结论
这些观察结果表明，膜通透性和功能的改变可能有助于 CAP 的抗增殖作用。
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