用扫描分光光度计测定血清两性霉素B
Serum amphotericin-B assay by scanning spectrophotometer.
作者信息
Shihabi Z K, Wasilauskas B L, Peacock J E
机构信息
Department of Pathology, Wake Forest University, Bowman Gray School of Medicine, Winston-Salem, North Carolina.
出版信息
Ther Drug Monit. 1988;10(4):486-9. doi: 10.1097/00007691-198804000-00020.
Abstract
We describe a simple, specific, 3-min assay for amphotericin-B in serum based on the absorbance at 408 nm and spectral scanning between 450 and 350 nm of the drug in acetonitrile extracts. The method correlated well with a high-performance liquid chromatographic method.
摘要
我们描述了一种基于乙腈提取物中药物在408nm处的吸光度以及450至350nm之间的光谱扫描，用于测定血清中两性霉素B的简单、特异的3分钟检测方法。该方法与高效液相色谱法相关性良好。
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