Department of Respiratory Medicine, the Fifth People's Hospital of Wuxi, Wuxi City, China.
J Clin Lab Anal. 2020 Jun;34(6):e23237. doi: 10.1002/jcla.23237. Epub 2020 Feb 14.
Deregulated circulating microRNAs (miRNAs) are potential biomarkers for the early detection and prognosis prediction of non-small-cell lung cancer (NSCLC). The aim of the present study was to investigate the expression pattern of serum exosomal miR-378 in NSCLC and its correlation with clinical variables.
Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was performed to detect serum exosomal miR-378 levels in 103 patients with NSCLC and 60 control subjects.
Our results showed that serum exosomal miR-378 was significantly overexpressed in NSCLC patients, and serum exosomal miR-378 upregulation was clearly associated with positive lymph node metastasis and advanced TNM stage. In addition, receiver operating characteristic (ROC) analysis demonstrated that combination of serum exosomal miR-378 expression and carcinoembryonic antigen (CEA) had a high discriminating power to differentiate NSCLC subjects from controls. Moreover, serum exosomal miR-378 levels in 73 NSCLC cases were significantly decreased after radiotherapy and could be used as an indicator of radiotherapeutic response in NSCLC. Furthermore, survival analyses revealed that patients with higher serum exosomal miR-378 expression had poor overall survival. Multivariate analysis showed that serum exosomal miR-378 expression was independently associated with overall survival.
Collectively, serum exosomal miR-378 has strong potential as a promising non-invasive biomarker for screening and monitoring NSCLC.
失调的循环 microRNAs（miRNAs）是用于非小细胞肺癌（NSCLC）早期检测和预后预测的潜在生物标志物。本研究旨在研究血清外泌体 miR-378 在 NSCLC 中的表达模式及其与临床变量的相关性。
采用定量逆转录聚合酶链反应（qRT-PCR）检测 103 例 NSCLC 患者和 60 例对照者血清外泌体 miR-378 水平。
我们的结果表明，NSCLC 患者血清外泌体 miR-378 明显过表达，血清外泌体 miR-378 的上调与阳性淋巴结转移和晚期 TNM 分期明显相关。此外，受试者工作特征（ROC）分析表明，血清外泌体 miR-378 表达与癌胚抗原（CEA）联合具有较高的鉴别能力，可区分 NSCLC 患者和对照组。此外，73 例 NSCLC 患者放疗后血清外泌体 miR-378 水平明显降低，可作为 NSCLC 放射治疗反应的指标。进一步的生存分析显示，血清外泌体 miR-378 表达较高的患者总生存率较差。多因素分析表明，血清外泌体 miR-378 表达与总生存率独立相关。
综上所述，血清外泌体 miR-378 具有作为 NSCLC 筛查和监测有前途的非侵入性生物标志物的强大潜力。
