Department of Neurology, No. 1 People's Hospital of Jining, Jining, Shandong, China.
Eur Rev Med Pharmacol Sci. 2020 Jan;24(2):837-842. doi: 10.26355/eurrev_202001_20067.
To investigate the effect of single nucleotide polymorphisms (SNPs) of signal transducer and activator of transcription 3 (STAT3) on epilepsy in children.
A total of 169 children suffering from epilepsy admitted in No. 1 People's Hospital of Jining from July 2015 to December 2016 were enrolled as the research subjects. Immunohistochemistry and real time-PCR were used for analysis of the expression of STAT3 and p-STAT3 in epilepsy patients. The genotypes and alleles of rs1053005 and re744166 were analyzed through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Statistical analysis was conducted to explore the correlation between the polymorphism of STAT3 and the incidence of epilepsy in children, and the polymorphism of STAT3 in the drug-resistant and non-resistant patients was compared.
Both the STAT3 and p-STAT3 were over-expressed in epilepsy patients. The GG genotype of rs1053005 was significantly lower in epilepsy patients than that of health control, p<0.05. By contrast, no significant difference was found in genotypes of rs744166 between epilepsy and healthy children. When comparing the genotypes of drug-resistant patients and that of non-resistant patients, the distribution of rs1053005 genotypes in the two groups showed a significant difference, p<0.05. No statistical difference was observed in rs744166 genotypes.
STAT3 polymorphism was associated with the risk of epilepsy and drug resistance to epilepsy. This study may provide a better understanding of STAT3 in epilepsy patients and provide new targets for the treatment of epilepsy patients.
研究信号转导子和转录激活子 3（STAT3）单核苷酸多态性（SNP）对儿童癫痫的影响。
选取 2015 年 7 月至 2016 年 12 月在济宁市第一人民医院就诊的 169 例癫痫患儿为研究对象，采用免疫组化和实时荧光定量 PCR 法分析癫痫患儿 STAT3 和 p-STAT3 的表达，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）法分析 STAT3 的 rs1053005 和 re744166 基因型和等位基因。统计分析探讨 STAT3 多态性与儿童癫痫发病的相关性，并比较 STAT3 多态性与耐药和非耐药患儿的关系。
癫痫患儿 STAT3 和 p-STAT3 均过表达。与健康对照组相比，癫痫患儿 rs1053005 的 GG 基因型明显降低，p<0.05。而癫痫患儿与健康儿童 rs744166 基因型无显著差异。耐药患儿与非耐药患儿 rs1053005 基因型分布比较，两组间基因型分布差异有统计学意义，p<0.05。rs744166 基因型在两组间无统计学差异。
STAT3 多态性与癫痫的发病风险和癫痫耐药有关。本研究可能为了解癫痫患者的 STAT3 提供更好的依据，并为癫痫患者的治疗提供新的靶点。
