Department of Periodontology and Research Institute of Oral Sciences, Gangneung-Wonju National University College of Dentistry, Gangneung, Korea.
Department of Anatomy and Research Institute of Oral Sciences, Gangneung-Wonju National University College of Dentistry, Gangneung, Korea.
J Periodontol. 2020 Oct;91(10):1367-1374. doi: 10.1002/JPER.19-0288. Epub 2020 Mar 16.
Periodontitis is associated with dysbiosis of microbial flora in the oral cavity. We evaluated the effects of an oral care probiotic, Weissella cibaria CMU, on periodontal tissue destruction and regulation of inflammatory cytokines in mice with ligature-induced periodontitis (LIP).
Fourteen-day LIP model was used. Ninety animals were randomly divided into six groups: negative control (Ctrl), positive control (LIP/Ctrl), PBS-treated (LIP/PBS), W. cibaria-low (1 × 10 colony forming unit (CFU)/d; LIP/WC-L), W. cibaria-medium (1 × 10 CFU/d; LIP/WC-M), and W. cibaria-high (1 × 10 CFU/d; LIP/WC-H). After the 14-day treatment, alveolar bone loss was determined using micro-computed tomography. The gingival tissue and serum samples from Ctrl, LIP/Ctrl, and LIP/WC-H groups were immunoassayed for cytokines. Measurements of Porphyromonas gingivalis, total bacteria, and W. cibaria in the gingiva were performed using real-time polymerase chain reaction.
Mice in the LIP/WC-H group showed significant reduction in alveolar bone loss at the distal aspect of the ligatured teeth compared to those in the LIP/Ctrl group. There was a dose-dependent reduction (non-significant) in periodontal tissue destruction with increased W. cibaria concentration. Pro- and anti-inflammatory cytokines were significantly lower in LIP/WC-H than in LIP/Ctrl. The LIP/WC-H group showed significantly fewer total bacteria compared to the LIP/Ctrl group but it was similar to that in Ctrl groups, and P. gingivalis was not detected in the gingival tissue.
W. cibaria CMU reduces periodontal tissue destruction apparently by regulating the production of inflammatory cytokines and by reducing oral bacteria in a model for periodontal disease.
牙周炎与口腔微生物菌群失调有关。我们评估了口腔保健益生菌韦荣球菌（Weissella cibaria）CMU 对结扎诱导牙周炎（LIP）小鼠牙周组织破坏和炎症细胞因子调节的影响。
使用 14 天 LIP 模型。90 只动物随机分为 6 组：阴性对照（Ctrl）、阳性对照（LIP/Ctrl）、PBS 处理（LIP/PBS）、韦荣球菌低剂量（1×10 菌落形成单位（CFU）/d；LIP/WC-L）、韦荣球菌中剂量（1×10 CFU/d；LIP/WC-M）和韦荣球菌高剂量（1×10 CFU/d；LIP/WC-H）。治疗 14 天后，使用微计算机断层扫描（micro-computed tomography）确定牙槽骨损失。Ctrl、LIP/Ctrl 和 LIP/WC-H 组的牙龈组织和血清样本进行细胞因子免疫测定。使用实时聚合酶链反应（real-time polymerase chain reaction）测量牙龈中的牙龈卟啉单胞菌、总细菌和韦荣球菌。
与 LIP/Ctrl 组相比，LIP/WC-H 组结扎牙远端牙槽骨丢失明显减少。随着韦荣球菌浓度的增加，牙周组织破坏呈剂量依赖性减少（无显著性）。LIP/WC-H 组促炎和抗炎细胞因子明显低于 LIP/Ctrl 组。LIP/WC-H 组总细菌数量明显少于 LIP/Ctrl 组，但与 Ctrl 组相似，牙龈组织中未检测到牙龈卟啉单胞菌。
韦荣球菌 CMU 通过调节炎症细胞因子的产生和减少牙周病模型中的口腔细菌，明显减少牙周组织破坏。
