Kang Mi-Sun, Park Geun-Yeong, Lee A-Reum
R&D Center, OraTicx, Inc., Seoul 04782, Republic of Korea.
Microorganisms. 2023 Apr 7;11(4):962. doi: 10.3390/microorganisms11040962.
In this study, we evaluated the in vitro anti-biofilm, antibacterial, and anti-inflammatory activity of CMU (CMU), an oral probiotic, against periodontopathogens. Compared to other oral probiotics, CMU showed a superior inhibitory effect on the biofilm formation and growth of on orthodontic wires and artificial teeth ( < 0.05). CMU exerted potent antibacterial effects against and according to a line test. In human gingival fibroblasts (HGFs) stimulated by , or , CMU suppressed the gene expression of pro-inflammatory cytokines [interleukin (IL)-6, IL-1β, IL-8, and tumor necrosis factor-α] in a dose-dependent manner ( < 0.05). CMU restored the production of the tissue inhibitor of metalloproteinase-1 following its inhibition by , and it suppressed the expression of matrix metalloproteinase (MMP)-1 and -3 induced by periodontopathogens ( < 0.05). Moreover, CMU needed direct contact with HGFs to exert their anti-inflammatory function, indicating that they act directly on gingival cells to modulate local inflammation. Our preclinical study provides evidence for the potential benefits of topical CMU treatments in preventing the development of caries and periodontitis caused by the dysbiosis of the dental plaque microbiome.
在本研究中，我们评估了口服益生菌CMU对牙周病原体的体外抗生物膜、抗菌和抗炎活性。与其他口服益生菌相比，CMU对正畸钢丝和人工牙齿上的生物膜形成和生长显示出更强的抑制作用（P<0.05）。根据划线试验，CMU对牙龈卟啉单胞菌和伴放线聚集杆菌具有强大的抗菌作用。在由脂多糖（LPS）或牙龈卟啉单胞菌刺激的人牙龈成纤维细胞（HGFs）中，CMU以剂量依赖性方式抑制促炎细胞因子[白细胞介素（IL）-6、IL-1β、IL-8和肿瘤坏死因子-α]的基因表达（P<0.05）。CMU在被LPS抑制后恢复了金属蛋白酶组织抑制剂-1的产生，并抑制了牙周病原体诱导的基质金属蛋白酶（MMP）-1和-3的表达（P<0.05）。此外，CMU需要与HGFs直接接触才能发挥其抗炎功能，这表明它们直接作用于牙龈细胞以调节局部炎症。我们的临床前研究为局部应用CMU治疗预防由牙菌斑微生物群失调引起的龋齿和牙周炎的潜在益处提供了证据。
