Department of Periodontology and Research Institute of Oral Sciences, Gangneung-Wonju National University College of Dentistry, Gangneung, Republic of Korea.
Department of Anatomy and Research Institute of Oral Sciences, Gangneung-Wonju National University College of Dentistry, Gangneung, Republic of Korea.
Oral Dis. 2024 Jul;30(5):3328-3335. doi: 10.1111/odi.14739. Epub 2023 Sep 19.
This study investigated the effects of Lactobacillus fermentum BELF11 on periodontitis in mice (LIP).
Sixty mice were randomly assigned to a control group (CTL), LIP/PBS group (LIP and PBS applied), or LIP/BELF11 group (LIP and L. fermentum BELF11 applied). For 14 days, PBS or L. fermentum BELF11 was applied twice daily to the mice in the LIP/PBS or LIP/BELF11 group, respectively. After 14 days, radiographic, histological, and pro-inflammatory cytokine assessments were conducted.
The LIP/PBS and LIP/BELF11 groups demonstrated greater alveolar bone loss than the CTL group (p < 0.05). The LIP/BELF11 group showed significantly reduced alveolar bone loss on the mesial side compared to the LIP/PBS group. Histologically, the LIP/BELF11 group showed consistent patterns of connective tissue fiber arrangement, lower levels of inflammatory infiltration, less alveolar bone loss, and higher alveolar bone density than the LIP/PBS group, despite showing more signs of destruction than the CTL group. The LIP/BELF11 group also exhibited significantly lower levels of pro-inflammatory cytokines than the LIP/PBS group.
L. fermentum BELF11 inhibits alveolar bone loss and periodontitis progression by regulating pro-inflammatory cytokine production. These findings suggest that L. fermentum BELF11 may be a potential adjunctive therapy in periodontal treatment.
本研究旨在探讨发酵乳杆菌 BELF11 对小鼠牙周炎（LIP）的影响。
将 60 只小鼠随机分为对照组（CTL）、LIP/PBS 组（LIP 和 PBS 处理）和 LIP/BELF11 组（LIP 和 L. fermentum BELF11 处理）。LIP/PBS 组和 LIP/BELF11 组小鼠分别每天接受两次 PBS 或 L. fermentum BELF11 处理，持续 14 天。14 天后，对小鼠进行放射学、组织学和促炎细胞因子评估。
与 CTL 组相比，LIP/PBS 组和 LIP/BELF11 组的牙槽骨丧失更为明显（p<0.05）。与 LIP/PBS 组相比，LIP/BELF11 组的近中侧牙槽骨丧失明显减少。组织学上，LIP/BELF11 组与 LIP/PBS 组相比，具有更一致的结缔组织纤维排列模式、更低水平的炎症浸润、较少的牙槽骨丧失和更高的牙槽骨密度，但与 CTL 组相比，仍显示出更多的破坏迹象。与 LIP/PBS 组相比，LIP/BELF11 组促炎细胞因子水平也显著降低。
L. fermentum BELF11 通过调节促炎细胞因子的产生，抑制牙槽骨丧失和牙周炎的进展。这些发现表明，L. fermentum BELF11 可能是牙周治疗的一种潜在辅助治疗方法。
