State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing, 211816, China.
Angew Chem Int Ed Engl. 2020 Apr 16;59(16):6428-6434. doi: 10.1002/anie.201915332. Epub 2020 Feb 25.
Hierarchically porous metal-organic frameworks (HP-MOFs) are promising in various applications. Most reported HP-MOFs are prepared based on the generation of mesopores in microporous frameworks, and the formed mesopores are connected by microporous channels, limiting the accessibility of mesopores for bulky molecules. A hierarchical structure is formed by constructing microporous MOFs in uninterrupted mesoporous tunnels. Using the confined space in as-prepared mesoporous silica, highly dispersed metal precursors for MOFs are coated on the internal surface of mesoporous tunnels. Ligand vapor-induced crystallization is employed to enable quantitative formation of MOFs in situ, in which sublimated ligands diffuse into mesoporous tunnels and react with metal precursors. The obtained hierarchically porous composites exhibit record-high adsorption capacity for the bulky molecule trypsin. The thermal and storage stability of trypsin is improved upon immobilization on the composites.
层级多孔金属有机骨架（HP-MOFs）在各种应用中具有广阔的前景。大多数已报道的 HP-MOFs 是通过在微孔骨架中生成介孔来制备的，形成的介孔通过微孔通道连接，限制了大体积分子进入介孔的通道。通过在连续的介孔隧道中构建微孔 MOFs 来形成分级结构。利用在介孔硅中预先形成的受限空间，将高度分散的 MOFs 金属前体涂覆在介孔隧道的内表面上。采用配体蒸气诱导结晶，定量原位生成 MOFs，其中升华的配体扩散进入介孔隧道并与金属前体反应。所得的分级多孔复合材料对大体积分子胰蛋白酶表现出创纪录的高吸附能力。固定在复合材料上后，胰蛋白酶的热稳定性和储存稳定性得到提高。
