The College of Forestry, Beijing Forestry University, Beijing, P.R. China.
College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin, P.R. China.
J Sep Sci. 2020 Apr;43(7):1339-1347. doi: 10.1002/jssc.201900754.
Taxanes are natural anticancer constituents, and the sample preparation from matrix normally depends on organochlorine solvents. In this study, green and natural menthol-based aqueous deep eutectic solvent was synthesized and used for sample preparation for taxanes. Five key parameters were optimized and the optimal preparation conditions were as follows: menthol/1-propanol ratio 1:1 (mol/mol), solid-liquid ratio 1:30 g/mL, extraction time 30 min, ultrasonic power 250 W, and water content 80%. Under the above conditions, the total extraction efficiency of seven main taxanes was 1.25- to 1.44-fold to the conventional methods. In addition, a high-performance liquid chromatography method with C column was established for quantitation of seven main taxanes in <25 min, which had excellent linearity (R > 0.9986), precision (relative standard deviation < 3.00%), repeatability (relative standard deviation < 3.69%), and recovery (90.26-109.00%). This method performed the extraction, and enrichment processes simultaneously, and it had advantages such as high extraction efficiency, simple operation, low cost, and eco-friendliness. This work indicated that the natural menthol-based deep eutectic solvent aqueous could be an excellent alternative to the sample preparation from Taxus or other plants.
紫杉醇是天然抗癌成分，其基质的样品制备通常依赖于有机氯溶剂。在本研究中，合成了基于天然薄荷醇的绿色和天然水基深共熔溶剂，并将其用于紫杉醇的样品制备。优化了五个关键参数，最佳制备条件如下：薄荷醇/1-丙醇比例为 1:1（摩尔比），固液比为 1:30 g/mL，提取时间 30 min，超声功率 250 W，含水量为 80%。在上述条件下，七种主要紫杉醇的总提取效率比常规方法提高了 1.25-1.44 倍。此外，还建立了一种在 25 min 内定量测定七种主要紫杉醇的高效液相色谱法，该方法具有优异的线性（R > 0.9986）、精密度（相对标准偏差 < 3.00%）、重复性（相对标准偏差 < 3.69%）和回收率（90.26-109.00%）。该方法同时进行了提取和富集过程，具有提取效率高、操作简单、成本低、环保等优点。本工作表明，天然薄荷醇基深共熔溶剂水相可以替代从紫杉或其他植物中制备样品。
