Department of Pharmacology and Toxicology, Faculty of Pharmacy, Comenius University in Bratislava, Bratislava, Slovak Republic.
COGnition and Action Group, UMR 8257, CNRS, Universite Paris Descartes, Paris, France.
Can J Physiol Pharmacol. 2020 Jul;98(7):473-476. doi: 10.1139/cjpp-2019-0565. Epub 2020 Feb 4.
Acetylcholine (ACh)-mediated vagal transmission as well as nonneuronal ACh release are considered cardioprotective in pathological situations with increased sympathetic drive such as ischemia-reperfusion and cardiac remodeling. ACh action is terminated by hydrolysis by the cholinesterases (ChEs), acetylcholinesterase, and butyrylcholinesterase. Both ChEs exist in multiple molecular variants either soluble or anchored by specific anchoring proteins like collagen Q (ColQ) anchoring protein and proline-rich membrane anchoring protein (PRiMA). Here we assessed the expression of specific ChE molecular forms in different heart compartments using RT-qPCR. We show that both ChEs are expressed in all heart compartments but display different expression patterns. The acetylcholinesterase-T variant together with PRiMA and ColQ is predominantly expressed in rat atria. Butylcholinesterase is found in all heart compartments and is accompanied by both PRiMA and ColQ anchors. Its expression in the ventricular system suggests involvement in the nonneuronal cholinergic system. Additionally, two PRiMA variants are detected throughout the rat heart.
乙酰胆碱（ACh）介导的迷走神经传递以及非神经元 ACh 释放被认为在交感神经驱动增加的病理情况下具有心脏保护作用，例如缺血再灌注和心脏重构。ACh 的作用通过胆碱酯酶（ChE）、乙酰胆碱酯酶和丁酰胆碱酯酶的水解而终止。这两种 ChE 都存在多种分子变体，要么是可溶性的，要么是通过特定的锚定蛋白（如胶原蛋白 Q（ColQ）锚定蛋白和富含脯氨酸的膜锚定蛋白（PRiMA））锚定的。在这里，我们使用 RT-qPCR 评估了不同心脏区室中特定 ChE 分子形式的表达。我们表明，两种 ChE 都在所有心脏区室中表达，但表达模式不同。乙酰胆碱酯酶-T 变体与 PRiMA 和 ColQ 一起主要在大鼠心房中表达。丁酰胆碱酯酶存在于所有心脏区室中，并伴有 PRiMA 和 ColQ 锚定。它在心室系统中的表达表明其参与了非神经元胆碱能系统。此外，还在整个大鼠心脏中检测到两种 PRiMA 变体。
