Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China.
Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China; College of Life Science, Shanxi University, Taiyuan, Shanxi 030006, China.
Int J Biol Macromol. 2020 Apr 15;149:741-753. doi: 10.1016/j.ijbiomac.2020.01.309. Epub 2020 Feb 1.
Cytochrome P450 monooxygenases (P450s) constitute a large superfamily of heme-thiolate proteins that are involved in the biosynthesis or degradation of endogenous compounds and detoxification of exogenous chemicals. It has been reported that P450s could serve as odorant-degrading enzymes (ODEs) to inactivate odorants to avoid saturating the antennae. However, there is little information about P450s in the antennae of Locusta migratoria. In the current work, we conducted an antenna transcriptome analysis and identified 92 P450s, including 68 full-length and 24 partial sequences. Phylogenetic analysis showed that 68 full-length P450s were grouped into four clans: CYP2, CYP3, CYP4, and mitochondria clans. Tissue, stage, and sex-dependent expressions of these 68 P450s were investigated. The results showed that 4 P450s were antenna-specific, whereas others were antenna-rich but also expressed in other tissues, implying their various potential roles in the antennae. In addition, the responses of seven selected P450s to five gramineous plant volatiles and four locust volatiles were determined. CYP6MU1 could be induced by almost all compounds tested, suggesting its important roles in odorant processing. Different P450s exhibited diverse responses to odorants, indicating that specific regulation of P450 expression by odorants might modulate the sensitivity of the olfactory responses to various chemicals.
细胞色素 P450 单加氧酶（P450s）构成了一个庞大的血红素硫醇蛋白超家族，参与内源性化合物的生物合成或降解以及外源性化学物质的解毒。据报道，P450s 可以作为气味降解酶（ODEs）来使气味失活，以避免使触角饱和。然而，关于 Locusta migratoria 触角中的 P450s 知之甚少。在目前的工作中，我们进行了触角转录组分析，鉴定了 92 种 P450s，包括 68 个全长和 24 个部分序列。系统发育分析表明，68 个全长 P450s 分为四个族：CYP2、CYP3、CYP4 和线粒体族。研究了这些 68 个 P450s 的组织、阶段和性别依赖性表达。结果表明，有 4 个 P450s 是触角特异性的，而其他的则是触角丰富的，但也在其他组织中表达，这表明它们在触角中具有不同的潜在作用。此外，还测定了七个选定的 P450s 对五种禾本科植物挥发物和四种蝗虫挥发物的反应。CYP6MU1 几乎可以被所有测试的化合物诱导，表明其在气味处理中起着重要作用。不同的 P450s 对气味表现出不同的反应，表明特定的 P450 表达调控可能通过气味调节对各种化学物质的嗅觉反应的敏感性。
