State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Scientific Observation and Experimental Station of Pests in Xilingol Rangeland, Ministry of Agriculture and Rural Affairs, Xilinhot, 026000, China.
Sci Rep. 2023 Mar 10;13(1):4048. doi: 10.1038/s41598-023-30889-1.
FK506 binding proteins (FKBPs) are a highly-conserved group of proteins known to bind to FK506, an immunosuppressive drug. They play different physiological roles, including transcription regulation, protein folding, signal transduction and immunosuppression. A number of FKBP genes have been identified in eukaryotes; however, very little information about these genes has been reported in Locusta migratoria. Here, we identified and characterized 10 FKBP genes from L. migratoria. Phylogenetic analysis and comparison of domain architectures indicated that the LmFKBP family can be divided into two subfamilies and five subclasses. Developmental and tissue expression pattern analysis revealed that all LmFKBPs transcripts, including LmFKBP46, LmFKBP12, LmFKBP47, LmFKBP79, LmFKBP16, LmFKBP24, LmFKBP44b, LmFKBP53, were periodically expressed during different developmental stages and mainly expressed in the fat body, hemolymph, testis, and ovary. In brief, our work depicts a outline but panoramic picture of LmFKBP family in L. migratoria, and provides a solid foundation to further investigate the molecular functions of LmFKBPs.
FK506 结合蛋白（FKBP）是一组高度保守的蛋白质，已知其与 FK506（一种免疫抑制剂）结合。它们发挥不同的生理作用，包括转录调控、蛋白质折叠、信号转导和免疫抑制。在真核生物中已经鉴定出许多 FKBP 基因，但在 Locusta migratoria 中关于这些基因的信息非常少。在这里，我们从 L. migratoria 中鉴定并表征了 10 个 FKBP 基因。系统发育分析和结构域比较表明，LmFKBP 家族可分为两个亚家族和五个亚类。发育和组织表达模式分析表明，所有 LmFKBPs 转录本，包括 LmFKBP46、LmFKBP12、LmFKBP47、LmFKBP79、LmFKBP16、LmFKBP24、LmFKBP44b、LmFKBP53，在不同的发育阶段周期性表达，主要在脂肪体、血液、睾丸和卵巢中表达。总之，我们的工作描绘了 L. migratoria 中 LmFKBP 家族的一个轮廓但全景图，并为进一步研究 LmFKBPs 的分子功能提供了坚实的基础。
