Institute of Bio- and Geosciences, IBG-2: Plant Sciences, Forschungszentrum Jülich GmbH, 52428 Jülich, Germany.
Biodiversity, University of Duisburg-Essen, Universitätsstr. 5, 45117 Essen, Germany.
Sensors (Basel). 2020 Jan 30;20(3):760. doi: 10.3390/s20030760.
We introduce a novel technique to measure volumes of any shaped objects based on acoustic components. The focus is on small objects with rough surfaces, such as plant seeds. The method allows measurement of object volumes more than 1000 times smaller than the volume of the sensor chamber with both high precision and high accuracy. The method is fast, noninvasive, and easy to produce and use. The measurement principle is supported by theory, describing the behavior of the measured data for objects of known volumes in a range of 1 to 800 µL. In addition to single-frequency, we present frequency-dependent measurements that provide supplementary information about pores on the surface of a measured object, such as the total volume of pores and, in the case of cylindrical pores, their average radius-to-length ratio. We demonstrate the usefulness of the method for seed phenotyping by measuring the volume of irregularly shaped seeds and showing the ability to "look" under the husk and inside pores, which allows us to assess the true density of seeds.
我们介绍了一种基于声学分量测量任意形状物体体积的新方法。该方法的重点是测量具有粗糙表面的小物体，如植物种子。该方法可以在比传感器腔体积小 1000 多倍的情况下进行测量，具有高精度和高准确性。该方法快速、非侵入式，易于制作和使用。测量原理得到了理论的支持，描述了在 1 到 800 µL 范围内具有已知体积的物体的测量数据的行为。除了单频测量，我们还展示了频率相关的测量结果，该结果提供了关于被测物体表面上的孔隙的补充信息，例如孔隙的总体积，以及在圆柱形孔隙的情况下，其平均半径与长度比。我们通过测量不规则形状种子的体积，并展示能够“透视”外壳和内部孔隙的能力，来证明该方法在种子表型分析中的有用性，这使我们能够评估种子的真实密度。
