苦味受体在非人类灵长类动物肠道细胞中的表达。
Expression of Bitter Taste Receptors in the Intestinal Cells of Non-Human Primates.
机构信息
Molecular Biology Section, Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Aichi 4848506, Japan.
Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, Tokyo 1568502, Japan.
出版信息
Int J Mol Sci. 2020 Jan 30;21(3):902. doi: 10.3390/ijms21030902.
(1) Background: Recent studies have investigated the expression of taste-related genes in the organs of various animals, including humans; however, data for additional taxa are needed to facilitate comparative analyses within and among species. (2) Methods: We investigated the expression of taste-related genes in the intestines of rhesus macaques, the non-human primates most commonly used in experimental models. (3) Results: Based on RNAseq and qRT-PCR, genes encoding bitter taste receptors and the G-protein gustducin were expressed in the gut of rhesus macaques. RNAscope analysis showed that one of the bitter receptors, TAS2R38, was expressed in some cells in the small intestine, and immunohistochemical analysis revealed the presence of T2R38-positive cells in the villi of the intestines. (4) Conclusions: These results suggest that bitter receptors are expressed in the gut of rhesus macaques, supporting the use of macaques as a model for studies of human taste, including gut analyses.
(1) 背景：最近的研究已经调查了味觉相关基因在包括人类在内的各种动物器官中的表达；然而，需要更多分类群的数据来促进种内和种间的比较分析。(2) 方法：我们调查了在猕猴的肠道中味觉相关基因的表达，猕猴是最常用于实验模型的非人类灵长类动物。(3) 结果：基于 RNAseq 和 qRT-PCR，编码苦味受体和 G 蛋白 gustducin 的基因在猕猴的肠道中表达。RNAscope 分析表明，一种苦味受体 TAS2R38 在小肠的一些细胞中表达，免疫组织化学分析显示 T2R38 阳性细胞存在于肠道的绒毛中。(4) 结论：这些结果表明，苦味受体在猕猴的肠道中表达，支持将猕猴用作人类味觉研究的模型，包括肠道分析。
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