Beijing advanced innovation center for food nutrition and human health, Beijing Technology and Business University, Beijing 100048, China.
College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
Molecules. 2020 Jan 30;25(3):610. doi: 10.3390/molecules25030610.
Astaxanthin-loaded liposomes were prepared by a thin-film ultrasonic method, and the effects of the different membrane surface modifiers chitosan hydrochloride (CH) and lactoferrin (LF) on the physicochemical stability of the liposomes and bioaccessibility of astaxanthin were studied. Based on the negative charge characteristics of egg yolk lecithin, LF and CH with positive charge were assembled on the surface of liposomes by an electrostatic deposition method. The optimal concentrations of modifiers were determined by particle size, zeta potential and encapsulation efficiency. The interaction between the liposomes and the coatings was characterized by Fourier Transform infrared spectroscopy. The stability of astaxanthin in different systems (suspension and liposomes) was investigated, and its antioxidant capacity and bioaccessibility were determined. The results showed that both membrane surface modifications could interact with liposomes and protect astaxanthin from oxidation or heat degradation and enhance the antioxidant activity of the liposome, therefore membrane surface modification played an important role in stabilizing the lipid bilayer. At the same time, the encapsulated astaxanthin exhibited higher bioaccessibility than the free astaxanthin. CH and LF modified liposomes can be developed as formulations for encapsulation and delivery of functional ingredients, providing a theoretical basis for the development of new astaxanthin series products.
用薄膜超声法制备虾青素脂质体，研究了不同膜表面修饰剂壳聚糖盐酸盐（CH）和乳铁蛋白（LF）对脂质体物理化学稳定性和虾青素生物利用度的影响。基于蛋黄卵磷脂的负电荷特性，通过静电沉积法将带正电荷的 LF 和 CH 组装在脂质体表面。通过粒径、Zeta 电位和包封效率确定了最佳的修饰剂浓度。通过傅里叶变换红外光谱对脂质体和涂层之间的相互作用进行了表征。研究了不同体系（悬浮液和脂质体）中虾青素的稳定性及其抗氧化能力和生物利用度。结果表明，两种膜表面修饰均可与脂质体相互作用，保护虾青素免受氧化或热降解，并增强脂质体的抗氧化活性，因此膜表面修饰在稳定脂质双分子层方面起着重要作用。同时，包封的虾青素表现出比游离虾青素更高的生物利用度。CH 和 LF 修饰的脂质体可用作功能性成分的包封和递送配方，为开发新的虾青素系列产品提供了理论依据。
