Sangsuriyawong Anantita, Limpawattana Maruj, Siriwan Dalad, Klaypradit Wanwimol
1Department of Fishery Products, Faculty of Fisheries, Kasetsart University, Bangkok, 10900 Thailand.
2Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok, Thailand.
Food Sci Biotechnol. 2018 Oct 30;28(2):529-537. doi: 10.1007/s10068-018-0495-x. eCollection 2019 Apr.
This study aimed to investigate the effects of phospholipid composition on the properties and bioavailability of astaxanthin-loaded liposomes using cell culture. Two mixtures of phospholipids with different proportions of phosphatidylcholine (PC, 23% and 70%) were used at various concentrations (0.8, 1.6, and 2.0% w/v) to prepare astaxanthin-loaded liposomes, which were investigated for entrapment efficiency (EE), antioxidant activity, morphology and changes in astaxanthin properties during a storage period of 8 weeks at 4 °C. Furthermore, Caco-2 human colon adenocarcinoma cells were employed to examine the cellular uptake of astaxanthin-loaded liposomes. The highest EE was observed with astaxanthin-loaded liposomes containing 70% PC, and used at the concentration of 2.0% w/v. Liposomes maintained the antioxidant activity of astaxanthin. All liposomal preparations were non-toxic. Cellular uptake of astaxanthin-loaded liposomes containing 70% PC was significantly higher than that of 23% PC-containing liposomes ( < 0.05).
本研究旨在通过细胞培养研究磷脂组成对载虾青素脂质体性质和生物利用度的影响。使用两种不同比例磷脂酰胆碱（PC，分别为23%和70%）的混合物，在不同浓度（0.8%、1.6%和2.0% w/v）下制备载虾青素脂质体，并对其包封率（EE）、抗氧化活性、形态以及在4℃下储存8周期间虾青素性质的变化进行研究。此外，采用Caco-2人结肠腺癌细胞来检测载虾青素脂质体的细胞摄取情况。含有70% PC且浓度为2.0% w/v的载虾青素脂质体的EE最高。脂质体保持了虾青素的抗氧化活性。所有脂质体制剂均无毒。含有70% PC的载虾青素脂质体的细胞摄取显著高于含有23% PC的脂质体（P < 0.05）。
