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长链非编码 RNA NNT-AS1 通过下调 miR-203 促进胆管癌细胞增殖和上皮-间充质转化。

Long non-coding RNA NNT-AS1 promotes cholangiocarcinoma cells proliferation and epithelial-to-mesenchymal transition through down-regulating miR-203.

机构信息

Emergency Intensive Care Unit, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.

Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.

出版信息

Aging (Albany NY). 2020 Feb 5;12(3):2333-2346. doi: 10.18632/aging.102747.

DOI:10.18632/aging.102747
PMID:32019904
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7041725/
Abstract

BACKGROUND

Cholangiocarcinoma (CCA) is a serious malignant tumor. Long non-coding RNA NNT-AS1 (NNT-AS1) takes crucial roles in several tumors. So, we planned to research the roles and underlying mechanism of NNT-AS1 in CCA.

RESULTS

NNT-AS1 overexpression was appeared in CCA tissues and cell lines. Proliferation was promoted by NNT-AS1 overexpression in CCLP1 and TFK1 cells. Besides, NNT-AS1 overexpression reduced E-cadherin level and raised levels of N-cadherin, vimentin, Snail and Slug. However, the opposite trend was occurred by NNT-AS1 knockdown. Further, NNT-AS1 overexpression promoted phosphatidylinositol 3 kinase (PI3K)/AKT and extracellular signal-regulated kinase (ERK)1/2 pathways. MiR-203 was sponged by NNT-AS1 and miR-203 mimic reversed the above promoting effects of NNT-AS1. Additionally, insulin-like growth factor type 1 receptor (IGF1R) and zinc finger E-box binding homeobox 1 (ZEB1) were two potential targets of miR-203.

CONCLUSION

NNT-AS1 promoted proliferation, EMT and PI3K/AKT and ERK1/2 pathways in CCLP1 and TFK1 cells through down-regulating miR-203.

METHODS

CCLP1 and TFK1 cells were co-transfected with pcDNA-NNT-AS1 and miR-203 mimic. Bromodeoxyuridine (BrdU), flow cytometry, quantitative reverse transcription polymerase chain reaction (qRT-PCR) and western blot were employed to detect roles and mechanism of NNT-AS1. Interaction between NNT-AS1 and miR-203 or miR-203 and target genes was examined through luciferase activity experiment.

摘要

背景

胆管癌（CCA）是一种严重的恶性肿瘤。长链非编码 RNA NNT-AS1（NNT-AS1）在几种肿瘤中发挥着关键作用。因此，我们计划研究 NNT-AS1 在 CCA 中的作用和潜在机制。

结果

NNT-AS1 在 CCA 组织和细胞系中呈现高表达。在 CCLP1 和 TFK1 细胞中，NNT-AS1 的过表达促进了细胞增殖。此外，NNT-AS1 的过表达降低了 E-钙黏蛋白的水平，提高了 N-钙黏蛋白、波形蛋白、Snail 和 Slug 的水平。然而，NNT-AS1 敲低则出现相反的趋势。进一步研究发现，NNT-AS1 的过表达促进了磷脂酰肌醇 3 激酶（PI3K）/蛋白激酶 B（AKT）和细胞外信号调节激酶（ERK）1/2 通路。NNT-AS1 可以吸附 miR-203，miR-203 模拟物逆转了 NNT-AS1 的上述促进作用。此外，胰岛素样生长因子 1 受体（IGF1R）和锌指 E 盒结合同源盒 1（ZEB1）是 miR-203 的两个潜在靶点。

结论

NNT-AS1 通过下调 miR-203 促进了 CCLP1 和 TFK1 细胞的增殖、上皮间质转化（EMT）以及 PI3K/AKT 和 ERK1/2 通路。

方法

用 pcDNA-NNT-AS1 和 miR-203 模拟物共转染 CCLP1 和 TFK1 细胞。采用溴脱氧尿苷（BrdU）掺入实验、流式细胞术、实时定量聚合酶链反应（qRT-PCR）和蛋白质印迹法检测 NNT-AS1 的作用和机制。通过荧光素酶活性实验检测 NNT-AS1 与 miR-203 或 miR-203 与靶基因之间的相互作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7145/7041725/aeaf859c44ed/aging-12-102747-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7145/7041725/798222dfc6d2/aging-12-102747-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7145/7041725/d61ed18d4f2b/aging-12-102747-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7145/7041725/704d92da8ccc/aging-12-102747-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7145/7041725/b6029021a37c/aging-12-102747-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7145/7041725/430340ccf775/aging-12-102747-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7145/7041725/926e33e15357/aging-12-102747-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7145/7041725/aeaf859c44ed/aging-12-102747-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7145/7041725/798222dfc6d2/aging-12-102747-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7145/7041725/d61ed18d4f2b/aging-12-102747-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7145/7041725/704d92da8ccc/aging-12-102747-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7145/7041725/b6029021a37c/aging-12-102747-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7145/7041725/430340ccf775/aging-12-102747-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7145/7041725/926e33e15357/aging-12-102747-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7145/7041725/aeaf859c44ed/aging-12-102747-g007.jpg

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本文引用的文献

1
MiR-203 acts as a radiosensitizer of gastric cancer cells by directly targeting ZEB1.微小RNA-203通过直接靶向锌指E盒结合蛋白1发挥胃癌细胞放射增敏剂的作用。
Onco Targets Ther. 2019 Jul 30;12:6093-6104. doi: 10.2147/OTT.S197539. eCollection 2019.
2
Up-regulation of microRNA-203 inhibits myocardial fibrosis and oxidative stress in mice with diabetic cardiomyopathy through the inhibition of PI3K/Akt signaling pathway via PIK3CA.miR-203 通过抑制 PIK3CA 对 PI3K/Akt 信号通路的抑制作用，上调抑制糖尿病心肌病小鼠心肌纤维化和氧化应激。
Gene. 2019 Oct 5;715:143995. doi: 10.1016/j.gene.2019.143995. Epub 2019 Jul 20.
3
20 个 miRNA 基因在卵巢癌扩散的各个步骤中的异常甲基化：从原发性肿瘤到腹膜宏观转移。
Int J Mol Sci. 2022 Jan 24;23(3):1300. doi: 10.3390/ijms23031300.
4
LncRNA NNT-AS1 regulates proliferation, ECM accumulation and inflammation of human mesangial cells induced by high glucose through miR-214-5p/smad4.长链非编码 RNA NNT-AS1 通过 miR-214-5p/smad4 调控高糖诱导的人肾小球系膜细胞增殖、细胞外基质积聚和炎症反应。
BMC Nephrol. 2021 Nov 6;22(1):368. doi: 10.1186/s12882-021-02580-y.
5
LncRNA-NNT-AS1 contributes to the progression of glioma by miR-582-5p/EZH2 axis.长链非编码RNA-NNT-AS1通过miR-582-5p/EZH2轴促进胶质瘤进展。
Cytotechnology. 2021 Jun;73(3):473-482. doi: 10.1007/s10616-021-00471-6. Epub 2021 Apr 28.
6
Overexpression of NNT-AS1 Activates TGF- Signaling to Decrease Tumor CD4 Lymphocyte Infiltration in Hepatocellular Carcinoma.NNT-AS1的过表达激活转化生长因子信号传导，以减少肝细胞癌中的肿瘤CD4淋巴细胞浸润。
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7
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8
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Cancers (Basel). 2020 Oct 23;12(11):3086. doi: 10.3390/cancers12113086.
9
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Aging (Albany NY). 2020 Sep 20;12(18):18571-18587. doi: 10.18632/aging.103849.
10
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BMC Cancer. 2020 Sep 14;20(1):879. doi: 10.1186/s12885-020-07348-5.
Upregulation of long non-coding RNA NNT-AS1 promotes osteosarcoma progression by inhibiting the tumor suppressive miR-320a.
长链非编码 RNA NNT-AS1 的上调通过抑制肿瘤抑制 miR-320a 促进骨肉瘤的进展。
Cancer Biol Ther. 2019;20(4):413-422. doi: 10.1080/15384047.2018.1538612. Epub 2018 Nov 29.
4
Long noncoding RNA NNT-AS1 promotes gastric cancer proliferation and invasion by regulating microRNA-363 expression.长链非编码 RNA NNT-AS1 通过调节 microRNA-363 的表达促进胃癌的增殖和侵袭。
J Cell Biochem. 2019 Apr;120(4):5704-5712. doi: 10.1002/jcb.27855. Epub 2018 Oct 15.
5
LncRNA NNT-AS1 promotes the proliferation, and invasion of lung cancer cells via regulating miR-129-5p expression.长链非编码 RNA NNT-AS1 通过调控 miR-129-5p 的表达促进肺癌细胞的增殖和侵袭。
Biomed Pharmacother. 2018 Sep;105:176-181. doi: 10.1016/j.biopha.2018.05.123. Epub 2018 May 29.
6
Long non-coding RNA NNT-AS1 affects progression of breast cancer through miR-142-3p/ZEB1 axis.长链非编码 RNA NNT-AS1 通过 miR-142-3p/ZEB1 轴影响乳腺癌的进展。
Biomed Pharmacother. 2018 Jul;103:939-946. doi: 10.1016/j.biopha.2018.04.087. Epub 2018 Apr 24.
7
Pleiotropic Roles for ZEB1 in Cancer.ZEB1 在癌症中的多效性作用。
Cancer Res. 2018 Jan 1;78(1):30-35. doi: 10.1158/0008-5472.CAN-17-2476. Epub 2017 Dec 18.
8
Long noncoding RNA NNT-AS1 promotes hepatocellular carcinoma progression and metastasis through miR-363/CDK6 axis.长链非编码RNA NNT-AS1通过miR-363/CDK6轴促进肝细胞癌的进展和转移。
Oncotarget. 2017 Sep 28;8(51):88804-88814. doi: 10.18632/oncotarget.21321. eCollection 2017 Oct 24.
9
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Cancer Sci. 2017 Dec;108(12):2366-2372. doi: 10.1111/cas.13401. Epub 2017 Oct 12.
10
Highly expressed long non-coding RNA NNT-AS1 promotes cell proliferation and invasion through Wnt/β-catenin signaling pathway in cervical cancer.高表达的长链非编码RNA NNT-AS1通过Wnt/β-连环蛋白信号通路促进宫颈癌细胞的增殖和侵袭。
Biomed Pharmacother. 2017 Aug;92:1128-1134. doi: 10.1016/j.biopha.2017.03.057. Epub 2017 Jun 13.