Pan Tonglin, Xue Min
The Department of Physiology, Xuzhou Medical University, 209th Tongshan Road, Xuzhou, 221004 Jiangsu Province People's Republic of China.
Cytotechnology. 2021 Jun;73(3):473-482. doi: 10.1007/s10616-021-00471-6. Epub 2021 Apr 28.
Increasing studies have shown that long non-coding RNAs (lncRNAs) had crucial regulatory roles in many diseases. Nevertheless, the biological relevance and mechanisms of the NNT-AS1 in gliom remain poorly understood. In the present study, NNT-AS1 expression was up-regulated in the glioma cell lines. Functional assays demonstrated that depletion of NNT-AS1 could notably suppress the proliferation, migration, invasion and EMT of U87MG and A172 cells. In addition, miR-582-5p was predicted to be a target of NNT-AS1 and EZH2 was predicted to be a target of miR-582-5p by bioinformatics software, which was further confirmed by luciferase reporter assay. Additionally, results of recue assays proofed that NNT-AS1/miR-582-5p/EZH2 axis aggravated the malignant behaviors of glioma. Ultimately, our findings revealed that NNT-AS1 contributes to progression via targeting miR-582-5p/EZH2 axis, revealing NNT-AS1 as a latent effective target for the diagnosis and treatment of glioma patients.
越来越多的研究表明，长链非编码RNA（lncRNAs）在许多疾病中发挥着关键的调控作用。然而，NNT-AS1在胶质瘤中的生物学相关性和机制仍知之甚少。在本研究中，胶质瘤细胞系中NNT-AS1表达上调。功能实验表明，敲低NNT-AS1可显著抑制U87MG和A172细胞的增殖、迁移、侵袭和上皮-间质转化。此外，生物信息学软件预测miR-582-5p是NNT-AS1的靶点，EZH2是miR-582-5p的靶点，荧光素酶报告基因实验进一步证实了这一点。此外，挽救实验结果证明NNT-AS1/miR-582-5p/EZH2轴加剧了胶质瘤的恶性行为。最终，我们的研究结果表明，NNT-AS1通过靶向miR-582-5p/EZH2轴促进进展，揭示了NNT-AS1作为胶质瘤患者诊断和治疗的潜在有效靶点。
