National Centre on Addiction and Doping, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome Italy.
Research Center in Health Sciences (CICS-UBI), University of Beira Interior, rua Marques de Avila e Bolama, 6299-001,Covilhã, Portugal.
J Anal Toxicol. 2020 Jul 31;44(6):570-579. doi: 10.1093/jat/bkz114.
Sampling and drug stability in oral fluid (OF) are crucial factors when interpreting forensic toxicological analysis, mainly because samples may not be analyzed immediately after collection, potentially altering drug concentrations. Therefore, the stability of some common drugs of abuse (morphine, codeine, 6-monoacetylmorphine, cocaine, benzoylecgonine, Δ9-tetrahydrocannabinol, cannabidiol, amphetamine, 3,4-methylenedioxymethamphetamine, ketamine) and the more commonly consumed new psychoactive substances in our environment (mephedrone, and N-(adamantan-1-yl)-1-(5-fluoropentyl)-1H-indazole-3-carboxamide 5F-AKB48 also known as 5F-APINACA) was investigated in an OF pool for the presence and absence of M3 Reagent Buffer® up to 1 year of storage. Fortified OF samples were stored at three different temperatures (room temperature, 4 and -20°C) to determine the best storage conditions over time. Control fortified OF samples were stored at -80°C for reference purposes. Compounds with concentration changes within ±15% of initial value were considered stable. The drugs were significantly more stable in M3 Reagent Buffer® than in neat OF samples in all storage conditions. All analytes were stable for 1 year at 4°C and -20°C in M3 Reagent Buffer®. Drugs stability in OF varied depending on the analyte, the presence of a stabilizer, the storage duration and temperature. When immediate sample analysis is not possible, we suggest to store OF samples at 4 or -20°C and test them within 2 weeks. Alternatively, OF samples may be stored at 4 or -20°C with M3 Reagent Buffer® to be tested within 1 year.
在解释法医毒理学分析结果时，唾液（OF）中的采样和药物稳定性是至关重要的因素，主要是因为采集后的样本可能不会立即进行分析，这可能会改变药物浓度。因此，研究了一些常见的滥用药物（吗啡、可待因、6-单乙酰吗啡、可卡因、苯甲酰爱康宁、Δ9-四氢大麻酚、大麻二酚、苯丙胺、3,4-亚甲二氧基甲基苯丙胺、氯胺酮）以及我们环境中更常见的新型精神活性物质（苯丙胺、和 N-（金刚烷-1-基）-1-（5-氟戊基）-1H-吲唑-3-甲酰胺 5F-AKB48 也称为 5F-APINACA）在添加或不添加 M3 试剂缓冲液®的情况下，在唾液池中储存长达 1 年的稳定性。将加标 OF 样品分别在三个不同温度（室温、4°C 和-20°C）下储存，以确定随着时间的推移最佳的储存条件。将对照加标 OF 样品储存在-80°C 以作参考。浓度变化在初始值的±15%范围内的化合物被认为是稳定的。在所有储存条件下，与未添加 M3 试剂缓冲液®的样品相比，添加 M3 试剂缓冲液®后的药物更稳定。所有分析物在 4°C 和-20°C 下在 M3 试剂缓冲液®中可稳定 1 年。在 M3 试剂缓冲液®中，药物在 OF 中的稳定性取决于分析物、稳定剂的存在、储存时间和温度。如果不能立即进行样品分析，我们建议将 OF 样品在 4°C 或-20°C 下储存，并在 2 周内进行测试。或者，也可以将 OF 样品在 4°C 或-20°C 下用 M3 试剂缓冲液®储存，并在 1 年内进行测试。
