Laboratory of Inorganic and Nuclear Chemistry, Wadsworth Center, New York State Department of Health, PO Box 509, Albany, NY, 12201, USA.
Department of Environmental Health Sciences, University at Albany, Albany, NY, 12222, USA.
Anal Bioanal Chem. 2020 Mar;412(8):1847-1861. doi: 10.1007/s00216-020-02432-y. Epub 2020 Feb 4.
Biomonitoring for human exposure to lead, arsenic, mercury, and other toxic metal(loid)s often relies on analyzing traditional biospecimens such as blood and urine. While biomonitoring based on blood and urine is well-established, non-traditional biospecimens such as hair and nails can offer the potential to explore past exposures as well as the advantages of non-invasive collection and ease of storage. The present study describes the production of four reference materials (NYS RMs 18-01 through 18-04) based on caprine horn, a keratinized tissue similar to human hair and nails, intended to serve as a resource for calibration, quality control, and method validation purposes. The elemental content and homogeneity of these candidate reference materials were characterized for 17 elements using inductively coupled plasma mass spectrometry (ICP-MS). Commutability between two or more of the NYS caprine horn RMs and human nails was established for 8 elements (Ba, Ca, Cr, Cu, Mn, Pb, Sr, and Zn) based on analysis by ICP-MS/MS and ICP-optical emission spectrometry. The development and optimization of an ICP-MS/MS instrumental method for the determination of 17 elements in keratinized tissues is described. The method was validated against three certified reference materials based on human hair showing good accuracy and method repeatability better than 25% for all analytes. This study also describes sample preparation issues and addresses common challenges including surface contamination, microwave digestion, matrix effects, and spectral interferences in inorganic mass spectrometry. New York State Department of Health Keratin Matrix Reference Materials. Graphical abstract.
生物监测是评估人类暴露于铅、砷、汞和其他有毒金属（类金属）的常用方法，通常依赖于分析血液和尿液等传统生物样本。虽然基于血液和尿液的生物监测已经得到广泛应用，但角蛋白组织（如头发和指甲）等非传统生物样本具有探索过去暴露情况的潜力，同时也具有非侵入式采集和易于储存的优势。本研究描述了四种角蛋白组织（NYS RMs 18-01 至 18-04）参考物质的制备，角蛋白组织类似于人类头发和指甲，旨在作为校准、质量控制和方法验证的资源。使用电感耦合等离子体质谱法（ICP-MS）对这些候选参考物质的 17 种元素的含量和均匀性进行了特征描述。通过 ICP-MS/MS 和 ICP-发射光谱法，对 NYS 角蛋白组织参考物质与人类指甲之间的两种或两种以上物质的可互换性进行了 8 种元素（Ba、Ca、Cr、Cu、Mn、Pb、Sr 和 Zn）的验证。描述了用于测定角蛋白组织中 17 种元素的 ICP-MS/MS 仪器方法的开发和优化。该方法针对三种基于人发的认证参考物质进行了验证，结果表明，所有分析物的准确性和方法重复性均良好，重复性优于 25%。本研究还描述了样品制备问题，并解决了常见的挑战，包括表面污染、微波消解、基质效应和无机质谱中的光谱干扰。
