Univ Rennes, Inserm, EHESP, Irset (Institut de recherche en santé, environnement et travail) - UMR_S 1085, Rennes, F-35043, France.
J Expo Sci Environ Epidemiol. 2019 Sep;29(5):706-717. doi: 10.1038/s41370-018-0112-3. Epub 2019 Jan 22.
Hair incorporates chemical compounds from the bloodstream and external sources as it grows. Different analytical procedures are proposed, but no consensus can be found for external contamination removal (washing stage). Thus, a major limitation of the use of hair analysis for human biomonitoring is the issue related to the washing efficiency, and the objective of this study was to propose a simple washing method for a better cleaning of external contamination. Based on a sequence of three steps of detergent or acid washing (Triton, nitric acid, and hydrochloric acid), the TNCl method was tested on raw and spiked samples and compared to other methods. Thirty-seven inorganic elements were analyzed by inductively Coupled Plasma Mass Spectrometry (ICP-MS) after washing and acid digestion of 10 hair samples (Li, Be, Na, Mg, Al, P, K, Ca, V, Cr, Fe, Mn, Co, N, Cu, Zn, As, Se, Sr, Mo, Ru, Ag, Cd, Sn, Sb, Cs, Ba, La, Ce, Nd, Gd, Lu, Tl, Pb, Bi, Th, and U). The inorganic element concentrations in the hair samples were compared to those reported in the literature. The TNCl method was shown to be more efficient than other methods based on the use of surfactants and organic solvents.
头发在生长过程中会从血液和外部来源中吸收化学物质。已经提出了不同的分析程序，但对于去除外部污染物（洗涤阶段）还没有达成共识。因此，使用头发分析进行人体生物监测的一个主要限制是与洗涤效率有关的问题，本研究的目的是提出一种简单的洗涤方法，以更好地去除外部污染物。基于 Triton、硝酸和盐酸的三步洗涤剂或酸洗涤（Triton、硝酸、盐酸）序列，测试了 TNCl 方法在原始和加标样品上的效果，并与其他方法进行了比较。在洗涤和 10 个头发样品的酸消解后，通过电感耦合等离子体质谱法（ICP-MS）分析了 37 种无机元素（Li、Be、Na、Mg、Al、P、K、Ca、V、Cr、Fe、Mn、Co、N、Cu、Zn、As、Se、Sr、Mo、Ru、Ag、Cd、Sn、Sb、Cs、Ba、La、Ce、Nd、Gd、Lu、Tl、Pb、Bi、Th 和 U）。将头发样品中的无机元素浓度与文献中的报道进行了比较。结果表明，与使用表面活性剂和有机溶剂的其他方法相比，TNCl 方法更为有效。
