Robert F. Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY, 14853, USA.
Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, CB3 0AS, UK.
Anal Bioanal Chem. 2020 Sep;412(24):6265-6273. doi: 10.1007/s00216-020-02449-3. Epub 2020 Feb 5.
We present a simple, rapid method for forming supported lipid bilayers on organic electronic devices composed of conducting polymer electrodes using a solvent-assisted lipid bilayer formation method. These supported bilayers present protein recognition elements that are mobile, critical for multivalent binding interactions. Because these polymers are transparent and conducting, we demonstrate, by optical and electrical detection, the specific interactions of proteins with these biomembrane-based bioelectronic devices. This work paves the way for easy formation of biomembrane mimetics for sensing and detection of binding events in a label-free manner on organic electronic devices of more sophisticated architectures. Graphical abstract.
我们提出了一种简单、快速的方法，通过溶剂辅助脂质双层形成方法，在由导电聚合物电极组成的有机电子器件上形成支撑脂质双层。这些支撑双层呈现出可移动的蛋白质识别元件，这对于多价结合相互作用至关重要。由于这些聚合物是透明的和导电的，我们通过光学和电学检测来证明蛋白质与这些基于生物膜的生物电子设备的特定相互作用。这项工作为在更复杂结构的有机电子设备上以无标记的方式感应和检测结合事件的生物膜模拟物的简单形成铺平了道路。
