Dall V, Luthman J
Acta Vet Scand. 1988;29(1):101-7. doi: 10.1186/BF03548398.
The damaging effect at the injection site (muscle) of a series of different preparations of penicillin C with increasing content of citrate buffer has been investigated in rabbits in order to improve the local tolerance of the drug preparation. The injections were given in M.sacrospinal and 3 days after injection the animals were sacrified and the areas of necrotic muscle tissue were isolated and weighed. The damage from the different preparations was determined on the basis of these weighing results. It was concluded that Novocillin 3.15 g and 6.3 g in 20 ml aqueous solution could be improved by adding 3x the original content of citrate buffer to the preparation resulting in a 38% decrease of the mean weight of necrotic areas at the injection site. Moreover, it was shown that the mean weight of necrotic areas at the injection site was diminished by 41% when injecting the improved 6.3 g concentration in a volume of 0.1 ml compared to injecting the improved 3.15 g concentration in a volume of 0.2 ml, which provides the same dose of penicillin G.
为提高药物制剂的局部耐受性，研究了一系列不同制剂的青霉素C（柠檬酸盐缓冲液含量不断增加）对家兔注射部位（肌肉）的损伤作用。在骶棘肌进行注射，注射3天后处死动物，分离并称重坏死肌肉组织的面积。根据这些称重结果确定不同制剂造成的损伤。得出结论，20ml水溶液中的3.15g和6.3g新青霉素，通过向制剂中添加3倍于原来含量的柠檬酸盐缓冲液可得到改善，这使得注射部位坏死区域的平均重量减少了38%。此外，结果表明，与以0.2ml体积注射改良后的3.15g浓度相比，以0.1ml体积注射改良后的6.3g浓度（二者青霉素G剂量相同）时，注射部位坏死区域的平均重量减少了41%。
