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Radioimmunoassay for luteinizing hormone in bovine species based on commercially available reagents.

作者信息

Høier R

出版信息

Acta Vet Scand. 1988;29(1):61-6. doi: 10.1186/BF03548393.

Abstract

A radioimmunoassay method based on commercially distributed antibody and antigen for measurement of bovine luteinizing hormone is presented. The method is characterized by (1) intraassay coefficient of variation (C.V.) from 1.3 to 10%, and (2) interassay C.V. from 4 to 18%. The specificity is illustrated by the cross reactivity of bFSH which is found to be 0.4%. The method provides good correlation with a previously well characterized method. Also the influence on sensitivity of length of incubation time is discussed.

摘要

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本文引用的文献

1
Progesterone and luteinizing hormone profiles in heifers used as oocyte donors.
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Measurement of specific radioactivities in labelled hormones by self-displacement analysis.
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Prediction of bovine ovulation by a rapid radioimmunoassay for plasma LH.
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Radioimmunoassay for bovine and ovine luteinizing hormone.
Endocrinology. 1969 May;84(5):1166-73. doi: 10.1210/endo-84-5-1166.
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Prostaglandins. 1975 Oct;10(4):675-84. doi: 10.1016/s0090-6980(75)80015-3.

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