基于市售试剂的牛促黄体生成素放射免疫分析
Radioimmunoassay for luteinizing hormone in bovine species based on commercially available reagents.
作者信息
Høier R
出版信息
Acta Vet Scand. 1988;29(1):61-6. doi: 10.1186/BF03548393.
Abstract
A radioimmunoassay method based on commercially distributed antibody and antigen for measurement of bovine luteinizing hormone is presented. The method is characterized by (1) intraassay coefficient of variation (C.V.) from 1.3 to 10%, and (2) interassay C.V. from 4 to 18%. The specificity is illustrated by the cross reactivity of bFSH which is found to be 0.4%. The method provides good correlation with a previously well characterized method. Also the influence on sensitivity of length of incubation time is discussed.
摘要
本文介绍了一种基于市售抗体和抗原的放射免疫分析方法，用于测定牛促黄体激素。该方法的特点是：（1）批内变异系数（C.V.）为1.3%至10%，（2）批间变异系数为4%至18%。通过检测发现，牛促卵泡素（bFSH）的交叉反应率为0.4%，表明该方法具有良好的特异性。该方法与之前一种特征明确的方法具有良好的相关性。此外，还讨论了孵育时间长短对灵敏度的影响。
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[Radioimmunologic determination of LH in bovine serum, with special attention to the estrous cycle].[牛血清中促黄体生成素的放射免疫测定，特别关注发情周期]
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