通过自置换分析法测定标记激素中的比放射性。
Measurement of specific radioactivities in labelled hormones by self-displacement analysis.
作者信息
Calvo J C, Radicella J P, Charreau E H
出版信息
Biochem J. 1983 May 15;212(2):259-64. doi: 10.1042/bj2120259.
Abstract
A graphical method is described that allows the determination of specific radioactivities of radioactively labelled hormones. This method combines the self-displacement technique, plotting bound/free ratios versus mass of unlabelled hormone or total radioactivity of labelled preparation added to the receptor preparation, and the maximal binding capacity of the labelled hormone. The procedure presented herein provides a more realistic specific radioactivity for use in all binding experiments. Application of the method is demonstrated for 125I-labelled ovine prolactin, and data are presented for 125I-labelled human choriogonadotropin and [3H]testosterone.
摘要
本文描述了一种用于测定放射性标记激素比活度的图解方法。该方法结合了自置换技术，即将结合/游离比与添加到受体制剂中的未标记激素质量或标记制剂的总放射性作图，以及标记激素的最大结合容量。本文介绍的方法可为所有结合实验提供更符合实际的比活度。文中展示了该方法在125I标记的绵羊催乳素上的应用，并给出了125I标记的人绒毛膜促性腺激素和[3H]睾酮的数据。
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本文引用的文献
1
Enzymatic iodination of polypeptides with 125I to high specific activity.用¹²⁵I对多肽进行酶促碘化以获得高比活度。
Biochim Biophys Acta. 1971 Dec 28;251(3):363-9. doi: 10.1016/0005-2795(71)90123-1.
2
Preparation of biologically active mono-125I-insulin of high specific activity.高比活生物活性单 - 125I胰岛素的制备
Acta Physiol Lat Am. 1981;31(1):13-24.
3
hCG-Leydig cell functional binding kinetics: threshold and nonlinearity in response.人绒毛膜促性腺激素-睾丸间质细胞功能结合动力学：反应阈值与非线性
Am J Physiol. 1980 Mar;238(3):E293-302. doi: 10.1152/ajpendo.1980.238.3.E293.
4
Gonadotroping binding and activation of the interstitial cells of the testis.促性腺激素与睾丸间质细胞的结合及激活。
Curr Top Mol Endocrinol. 1974;1:1-30. doi: 10.1007/978-1-4684-2595-6_1.
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