Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, Saudi Arabia.
J Biochem Mol Toxicol. 2020 Apr;34(4):e22456. doi: 10.1002/jbt.22456. Epub 2020 Feb 5.
The current examination was intended to observe the defensive impacts of embelin against paraquat-incited lung damage in relationship with its antioxidant and anti-inflammatory action. Oxidative stress marker, like malondialdehyde (MDA), antioxidative enzymes, for example, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH Px), inflammatory cytokines, such as interleukin-1β (IL-1β), tumor necrosis factor-α, and IL-6, histological examination, and nuclear factor kappa B/mitogen-activated protein kinase (NF-κB/MAPK) gene expression were evaluated in lung tissue. Embelin treatment significantly decreased MDA and increased SOD, CAT, and GSH Px. Embelin significantly reduced levels of inflammatory cytokines in paraquat-administered and paraquat-intoxicated rats. In addition, embelin suggestively decreased relative protein expression of nuclear NF-κB p65, p-NF-κBp65, p38 MAPK, and p-p38 MAPKs in paraquat-intoxicated rats. The outcomes show the impact of embelin inhibitory action on NF-κB and MAPK and inflammatory cytokines release, and the decrease of lung tissue damage caused by paraquat.
本次检查旨在观察棓丙酯通过抗氧化和抗炎作用对百草枯引起的肺损伤的保护作用。在肺组织中评估了氧化应激标志物，如丙二醛（MDA），抗氧化酶，如超氧化物歧化酶（SOD），过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH Px），炎性细胞因子，如白细胞介素-1β（IL-1β），肿瘤坏死因子-α和 IL-6，组织学检查和核因子 kappa B/丝裂原激活蛋白激酶（NF-κB/MAPK）基因表达。棓丙酯治疗可显著降低 MDA 并增加 SOD、CAT 和 GSH Px。棓丙酯可显著降低百草枯给药和百草枯中毒大鼠的炎性细胞因子水平。此外，棓丙酯可降低百草枯中毒大鼠核 NF-κB p65、p-NF-κBp65、p38 MAPK 和 p-p38 MAPKs 的相对蛋白表达。结果表明棓丙酯对 NF-κB 和 MAPK 及炎性细胞因子释放的抑制作用以及百草枯引起的肺组织损伤的减少。
