Beijing University of Chinese Medicine, State Administration of Traditional Chinese Medicine, Research Center of TCM-Information Engineering, Beijing, 100102, China.
BMC Complement Med Ther. 2020 Feb 3;20(1):28. doi: 10.1186/s12906-019-2712-4.
The tanshinones and phenolic acids in Salvia miltiorrhiza (also named Danshen) have been confirmed for the treatment of coronary heart disease (CHD), but the action mechanisms remain elusive.
In the current study, the co-expression protein interaction network (Ce-PIN) was used to illustrate the differences between the tanshinones and phenolic acids of Danshen in the treatment of CHD. By integrating the gene expression profile data and protein-protein interactions (PPIs) data, the Ce-PINs of tanshinones and phenolic acids were constructed. Then, the Ce-PINs were analyzed by gene ontology enrichment analyzed based on the optimal algorithm.
It turned out that Danshen is able to treat CHD by regulating the blood circulation, immune response and lipid metabolism. However, phenolic acids may regulate the blood circulation by Extracellular calcium-sensing receptor (CaSR), Endothelin-1 receptor (EDNRA), Endothelin-1 receptor (EDNRB), Kininogen-1 (KNG1), tanshinones may regulate the blood circulation by Guanylate cyclase soluble subunit alpha-1 (GUCY1A3) and Guanylate cyclase soluble subunit beta-1 (GUCY1B3). In addition, both the phenolic acids and tanshinones may regulate the immune response or inflammation by T-cell surface glycoprotein CD4 (CD4), Receptor-type tyrosine-protein phosphatase C (PTPRC).
Through the same targets of the same biological process and different targets of the same biological process, the tanshinones and phenolic acids synergistically treat coronary heart disease.
丹参中的丹参酮和酚酸已被证实可用于治疗冠心病（CHD），但其作用机制仍不清楚。
在本研究中，共表达蛋白相互作用网络（Ce-PIN）用于说明丹参中丹参酮和酚酸在治疗 CHD 方面的差异。通过整合基因表达谱数据和蛋白质-蛋白质相互作用（PPIs）数据，构建了丹参酮和酚酸的 Ce-PINs。然后，基于最优算法，对 Ce-PINs 进行基因本体论富集分析。
结果表明，丹参通过调节血液循环、免疫反应和脂质代谢来治疗 CHD。然而，酚酸可能通过细胞外钙敏感受体（CaSR）、内皮素 1 受体（EDNRA）、内皮素 1 受体（EDNRB）、激肽原 1（KNG1）来调节血液循环，丹参酮可能通过可溶性鸟苷酸环化酶亚基α-1（GUCY1A3）和可溶性鸟苷酸环化酶亚基β-1（GUCY1B3）来调节血液循环。此外，酚酸和丹参酮可能通过 T 细胞表面糖蛋白 CD4（CD4）、受体型酪氨酸蛋白磷酸酶 C（PTPRC）共同调节免疫反应或炎症。
丹参酮和酚酸通过相同生物过程的相同靶点和相同生物过程的不同靶点协同治疗冠心病。
