Changchun University of Chinese Medicine, Boshuo Road No. 1035, Jingyue High-tech Industrial Development Zone, Changchun, 130117, China.
BMC Complement Med Ther. 2020 Jan 15;20(1):7. doi: 10.1186/s12906-019-2785-0.
As the dry rhizome of Anemone raddeana Regel, Rhizoma Anemones Raddeanae (RAR), which belongs to Ranunculaceae, is usually used to treat wind and cold symptoms, hand-foot disease and spasms, joint pain and ulcer pain in China. It is well known that the efficacy of RAR can be distinctly enhanced by processing with vinegar due to the reduced toxicity and side effects. However, the entry of vinegar into liver channels can cause a series of problems. In this paper, the differences in the acute toxicity, anti-inflammatory and analgesic effects between RAR and vinegar-processed RAR were compared in detail. The changes in the chemical compositions between RAR and vinegar-processed RAR were investigated, and the mechanism of vinegar processing was also explored.
Acute toxicity experiments were used to examine the toxicity of vinegar-processed RAR. A series of studies, such as the writhing reaction, ear swelling experiment, complete Freund's adjuvant-induced rat foot swelling experiment and cotton granuloma, in experimental mice was conducted to observe the anti-inflammatory effect of vinegar-processed RAR. The inflammatory cytokines of model rats were determined by enzyme-linked immunosorbent assay (ELISA). Liquid Chromatography-Quadrupole-Time of Flight mass spectrometer Detector (LC-Q-TOF) was used to analyse the chemical compositions of the RARs before and after vinegar processing.
Neither obvious changes in mice nor death phenomena were observed as the amount of vinegar-processed RAR in crude drug was set at 2.1 g/kg. Vinegar-processed RAR could significantly prolong the latency, reduce the writhing reaction time to reduce the severity of ear swelling and foot swelling, and remarkably inhibit the secretion of Interleukin-1β(IL-1β), Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) proinflammatory cytokines. The content of twelve saponins (e.g., Eleutheroside K) in RAR was decreased after vinegar processing, but six other types (e.g., RDA) were increased.
These results revealed that vinegar processing could not only improve the analgesic and anti-inflammatory effects of RAR but also reduce its own toxicity.
Not applicable.
作为毛茛科升麻属植物升麻的干燥根茎，中药升麻（RAR）通常用于治疗风寒、手足病、痉挛、关节痛和溃疡痛。众所周知，由于毒性和副作用降低，醋处理可以明显增强 RAR 的功效。然而，醋进入肝经会引起一系列问题。本文详细比较了 RAR 和醋处理的 RAR 的急性毒性、抗炎和镇痛作用的差异。研究了 RAR 和醋处理的 RAR 之间化学成分的变化，并探讨了醋处理的机制。
急性毒性实验用于检测醋处理的 RAR 的毒性。在实验小鼠中进行了一系列研究，如扭体反应、耳肿胀实验、完全弗氏佐剂诱导的大鼠足肿胀实验和棉肉芽肿，观察醋处理的 RAR 的抗炎作用。通过酶联免疫吸附试验（ELISA）测定模型大鼠的炎症细胞因子。采用液相色谱-四极杆飞行时间质谱联用仪（LC-Q-TOF）分析醋处理前后 RAR 的化学成分。
当生药中醋处理的 RAR 量设定为 2.1g/kg 时，在小鼠中既没有观察到明显的变化，也没有观察到死亡现象。醋处理的 RAR 能显著延长潜伏期，减少扭体反应时间，减轻耳肿胀和足肿胀的严重程度，显著抑制白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）促炎细胞因子的分泌。醋处理后，RAR 中的 12 种皂苷（如 Eleutheroside K）含量降低，但其他 6 种（如 RDA）含量增加。
这些结果表明，醋处理不仅可以提高 RAR 的镇痛和抗炎作用，而且可以降低其自身的毒性。
不适用。
