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长链非编码RNA FLVCR-AS1通过靶向miR381-3p/CCND1促进骨肉瘤生长。

lncRNA FLVCR-AS1 promotes osteosarcoma growth by targeting miR381-3p/CCND1.

作者信息

Yang Guang, He Fei, Duan Hao, Shen Jianlin, Dong Qirong

机构信息

Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.

Department of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming 650200, China.

出版信息

Onco Targets Ther. 2020 Jan 9;13:163-172. doi: 10.2147/OTT.S214813. eCollection 2020.

DOI:10.2147/OTT.S214813
PMID:32021264
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6966140/
Abstract

PURPOSE

This article reports on FLVCR-AS1 effects on osteosarcoma (OS) growth.

METHODS

Tumor tissue and adjacent normal tissue of 48 OS patients were collected. HOS and 143B cells were transfected. Gene expression was examined with qRT-PCR and Western blot. CCK8 assays and cell cloning was performed to measure cell proliferation. Cell cycle and apoptosis were assessed. Luciferase-reporter gene assays and RNA pull-down tests were used to detect targeting relationships between genes.

RESULTS

Prominently higher FLVCR-AS1 expression was found in OS tissue and cells, and was associated with poor prognosis (<0.05, <0.01, or <0.001). Compared with the siCtrl group, 143B and HOS cells of the siFLVCR-AS1 group had significantly lower OD values and clone numbers and obviously higher percentages of cells in the G phase and apoptosis (<0.01 or <0.001). miR381-3p expression was directly inhibited by FLVCR-AS1, and CCND1 expression was directly suppressed by miR381-3p. Compared with the FLVCR-AS1 group, 143B cells of the FLVCR-AS1 miR381-3p mimic group and FLVCR-AS1 siCCND1 group showed remarkably lower OD values and clone numbers obviously higher apoptosis and percentage of cells in the G phase (<0.05, <0.01, or <0.001).

CONCLUSION

FLVCR-AS1 promoted OS growth by upregulating CCND1 expression via downregulation of miR381-3p.

摘要

目的

本文报道了FLVCR-AS1对骨肉瘤（OS）生长的影响。

方法

收集48例OS患者的肿瘤组织及相邻正常组织。对HOS和143B细胞进行转染。采用qRT-PCR和蛋白质免疫印迹法检测基因表达。进行CCK8检测和细胞克隆以测量细胞增殖。评估细胞周期和细胞凋亡。采用荧光素酶报告基因检测和RNA下拉试验检测基因之间的靶向关系。

结果

在OS组织和细胞中发现FLVCR-AS1表达显著更高，且与预后不良相关（<0.05、<0.01或<0.001）。与siCtrl组相比，siFLVCR-AS1组的143B和HOS细胞的OD值和克隆数显著更低，G期细胞百分比和凋亡率明显更高（<0.01或<0.001）。FLVCR-AS1直接抑制miR381-3p表达，miR381-3p直接抑制CCND1表达。与FLVCR-AS1组相比，FLVCR-AS1 miR381-3p模拟物组和FLVCR-AS1 siCCND1组的143B细胞的OD值和克隆数显著更低，凋亡率和G期细胞百分比明显更高（<0.05、<0.01或<0.001）。

结论

FLVCR-AS1通过下调miR381-3p上调CCND1表达促进OS生长。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/383f/6966140/ba94f5aed2eb/OTT-13-163-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/383f/6966140/347ce2f760c6/OTT-13-163-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/383f/6966140/f0cc4ea59773/OTT-13-163-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/383f/6966140/6cca67ff583c/OTT-13-163-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/383f/6966140/06bd8e6c3083/OTT-13-163-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/383f/6966140/ba94f5aed2eb/OTT-13-163-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/383f/6966140/347ce2f760c6/OTT-13-163-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/383f/6966140/f0cc4ea59773/OTT-13-163-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/383f/6966140/6cca67ff583c/OTT-13-163-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/383f/6966140/06bd8e6c3083/OTT-13-163-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/383f/6966140/ba94f5aed2eb/OTT-13-163-g0005.jpg

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