通过比率荧光探针观察 5-氟尿嘧啶治疗过程中细胞内过氧亚硝酸盐的产生。
Observation of peroxynitrite overproduction in cells during 5-fluorouracil treatment via a ratiometric fluorescent probe.
机构信息
College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, P. R. China.
出版信息
Chem Commun (Camb). 2020 Mar 4;56(18):2759-2762. doi: 10.1039/c9cc09652c. Epub 2020 Feb 5.
PMID:32022003
Abstract
We describe a colorimetric and fluorescent probe 3a to detect cellular peroxynitrite with high selectivity and sensitivity. 3a was successfully applied in the bioimaging of exogenous and endogenous peroxynitrite in living cells. The up-regulation of peroxynitrite in cancer cells and normal cells during 5-fluorouracil treatment was finally monitored.
摘要
我们描述了一种比色和荧光探针 3a，它可以高选择性和灵敏度地检测细胞中的过氧亚硝酸盐。3a 成功地应用于活细胞中外源和内源性过氧亚硝酸盐的生物成像。最后监测了氟尿嘧啶治疗过程中癌细胞和正常细胞中过氧亚硝酸盐的上调。
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