Santner S J, Kulin H E, Santen R J
J Clin Endocrinol Metab. 1977 Feb;44(2):313-21. doi: 10.1210/jcem-44-2-313.
Multiple blood sampling techniques and short-term, timed urine collections were employed before and after luteinizing hormone releasing factor (LRF) administration to 51 individuals on 58 occasions. Correlation of blood and urine per cent responses to LRF were significant for LH (P less than .05) and FSH (P less than .001), indicating that urine measurements provide an adequate means of assessing response to LRF. In 7 patients with basal blood gonadotropin levels below assay sensitivity, urine measurements provided the only means of accurately determining a response to LRF. Per cent response to LRF was negatively correlated with basal LH levels in the urine (P less than .02) but not in the blood. A significant negative correlation between basal levels and per cent response was demonstrated for FSH in blood and urine (P less than .01). Accurate measurement of basal gonadotropins and the expression of LRF responses as per cent increments aided in distinguishing between patients with hypothalamic and pituitary diseases. A marked response to LRF in the presence of very low basal LH levels was found in patients with hypothalamic disorders, a finding revealed only by using urine determinations. Low per cent responses to LRF were seen primarily in patients with pituitary disease, a situation most clearly delineated by blood FSH measurements.
在58个不同场合，对51名个体施用促黄体生成激素释放因子（LRF）前后，采用了多种采血技术和短期定时尿液收集方法。血液和尿液对LRF的反应百分比之间，促黄体生成素（LH）（P<0.05）和促卵泡生成素（FSH）（P<0.001）的相关性显著，表明尿液测量提供了评估对LRF反应的充分手段。在7名基础血促性腺激素水平低于检测灵敏度的患者中，尿液测量是准确确定对LRF反应的唯一手段。尿液中对LRF的反应百分比与基础LH水平呈负相关（P<0.02），但血液中无此相关性。血液和尿液中FSH的基础水平与反应百分比之间存在显著负相关（P<0.01）。准确测量基础促性腺激素并将LRF反应表示为百分比增量，有助于区分下丘脑和垂体疾病患者。下丘脑疾病患者在基础LH水平极低的情况下对LRF有明显反应，这一发现仅通过尿液测定得以揭示。对LRF反应百分比低主要见于垂体疾病患者，这种情况通过血液FSH测量最为清晰地界定。
