Department of Vascular and Endovascular Surgery, First Affiliated Hospital of Zhengzhou University, Henan, China.
Key Vascular Physiology and Applied Research Laboratory of Zhengzhou City, Henan, China.
J Biomed Mater Res B Appl Biomater. 2020 Aug;108(6):2417-2425. doi: 10.1002/jbm.b.34574. Epub 2020 Feb 5.
Although the science of implantable materials has advanced therapeutic options in vascular surgery, graft failure is still a problem in need of a durable solution. With the development of coating and decellularization techniques, coated prosthetic grafts have become an option; however, whether decellularized human saphenous vein can be conjugated and implanted is not known. Human great saphenous vein (GSV) was harvested and decellularized and hyaluronic acid (HA)-heparin was conjugated to the GSV; water contact angles (WCA), morphology, and sulfur element change were measured before and after heparin bonding. GSV patches were implanted into the rat inferior vena cava and aorta; patches were harvested (Day 14) and analyzed. HA-heparin was successfully conjugated to the decellularized human GSV with altered morphology and reduced WCA. The HA-heparin coated decellularized GSV patch was anti-thrombotic in vitro, and significantly decreased neointimal thickness both in patch venoplasty and angioplasty in a rat model. Both CD90 and nestin positive cells participated in neointima formation. These data show that HA-heparin coated human GSV patches decrease neointimal thickness when used both in venoplasty and arterioplasty. Tissue engineered decellularized human GSV is a promising vascular prosthesis.
虽然植入材料科学为血管外科学带来了更先进的治疗选择，但移植物失败仍然是一个需要持久解决方案的问题。随着涂层和脱细胞技术的发展，涂层假体移植物已成为一种选择；然而，脱细胞的人隐静脉是否可以被结合并植入仍然未知。从人体中采集并脱细胞的大隐静脉（GSV），并将透明质酸（HA）-肝素结合到 GSV 上；在肝素结合前后测量水接触角（WCA）、形态和硫元素变化。将 GSV 贴片植入大鼠下腔静脉和主动脉；在第 14 天取出并进行分析。HA-肝素成功地与脱细胞的人 GSV 结合，改变了形态并降低了 WCA。体外研究表明，HA-肝素涂层的脱细胞 GSV 贴片具有抗血栓作用，在大鼠模型中，无论是在静脉成形术还是血管成形术中，都能显著降低新生内膜厚度。CD90 和巢蛋白阳性细胞均参与了新生内膜的形成。这些数据表明，HA-肝素涂层的人 GSV 贴片在静脉成形术和动脉成形术中都能降低新生内膜厚度。组织工程化脱细胞人 GSV 是一种有前途的血管假体。
