Sun Peng, Yan Shujie, Zhang Liwei, Zhang Cong, Wu Haoliang, Wei Shunbo, Xie Boao, Wang Xiaofeng, Bai Hualong
Department of Vascular and Endovascular Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Key Vascular Physiology and Applied Research Laboratory of Zhengzhou City, Zhengzhou, China.
Front Bioeng Biotechnol. 2022 Mar 18;10:843590. doi: 10.3389/fbioe.2022.843590. eCollection 2022.
The egg shell membrane (ESM) is always considered as waste, but recent studies have shown that it has the potential to yield rapid re-endothelialization . We hypothesized that ESM and heparin-conjugated ESM (HESM) can be used as arterial patch in a rat aortic angioplasty model. Sprague-Dawley rat (200 g) abdominal aortic patch angioplasty model was used. Decellularized rat thoracic aorta (TA) patch was used as the control; ESM patch was made of raw chicken egg; heparin-coated ESM (HESM) patch was made by using dopamine; anticoagulation properties were verified using platelet adhesion tests; the TA, ESM, and HESM patches were implanted to the rat aorta and harvested at day 14; and the samples were examined by immunohistochemistry and immunofluorescence. The ESM patch showed a similar healing process to the TA patch; the cells could migrate and infiltrate into both patches; there was a neointima with von Willebrand factor-positive endothelial cells; the endothelial cells acquired arterial identity with Ephrin-B2- and dll-4-positive cells; there were proliferating cell nuclear antigen (PCNA)-positive cells, and PCNA and alpha smooth muscle actin dual-positive cells in the neointima in both groups. Heparin was conjugated to the patch successfully and showed a strong anticoagulation property . HESM could decrease mural thrombus formation after rat aortic patch angioplasty. The ESM is a natural scaffold that can be used as a vascular patch; it showed a similar healing process to decellularized TA patch; HESM showed anticoagulation property both and ; and the ESM may be a promising vascular graft in the clinic.
卵壳膜（ESM）一直被视为废弃物，但最近的研究表明，它具有促进快速再内皮化的潜力。我们推测，ESM和肝素共轭ESM（HESM）可用于大鼠主动脉血管成形术模型中的动脉补片。采用Sprague-Dawley大鼠（200 g）腹主动脉补片血管成形术模型。脱细胞大鼠胸主动脉（TA）补片用作对照；ESM补片由生鸡蛋制成；肝素包被的ESM（HESM）补片通过使用多巴胺制成；通过血小板黏附试验验证抗凝性能；将TA、ESM和HESM补片植入大鼠主动脉，并在第14天收获；通过免疫组织化学和免疫荧光检查样本。ESM补片显示出与TA补片相似的愈合过程；细胞可以迁移并浸润到两个补片中；有一个含有血管性血友病因子阳性内皮细胞的新生内膜；内皮细胞通过Ephrin-B2和dll-4阳性细胞获得动脉特征；两组新生内膜中均有增殖细胞核抗原（PCNA）阳性细胞，以及PCNA和α平滑肌肌动蛋白双阳性细胞。肝素成功地与补片结合，并显示出强大的抗凝性能。HESM可减少大鼠主动脉补片血管成形术后壁血栓形成。ESM是一种天然支架，可作为血管补片使用；它显示出与脱细胞TA补片相似的愈合过程；HESM在体内和体外均显示出抗凝性能；并且ESM可能是临床上一种有前途的血管移植物。
