Bai Hualong, Li Xin, Hashimoto Takuya, Hu Haidi, Foster Trenton R, Hanisch Jesse J, Santana Jeans M, Dardik Alan
Department of Surgery, Yale University; Vascular Biology and Therapeutics Program, Yale University; Department of Vascular Surgery, First Affiliated Hospital of Zhengzhou University; Basic Medical College of Zhengzhou University; VA Connecticut Healthcare Systems, West Haven, CT.
Department of Vascular Surgery, Xiangya Second Hospital of Central South University, Changsha, China.
J Vis Exp. 2017 Feb 27(120):55253. doi: 10.3791/55253.
Pericardial patches are commonly used in vascular surgery to close vessels. To facilitate studies of the neointimal hyperplasia that forms on the patch, we developed a rat model of patch angioplasty that can be used in either a vein or an artery, creating a patch venoplasty or a patch arterioplasty, respectively. Technical aspects of this model are discussed. The infra-renal IVC or aorta are dissected and then clamped proximally and distally. A 3 mm venotomy or arteriotomy is performed in the infrarenal inferior vena cava or aorta of 6 to 8 week-old Wistar rats. A bovine pericardial patch (3 mm x 1.5 mm x 0.6 mm) is then used to close the site using a 10-0 nylon suture. Compared to arterial patches, venous patches show increased neointimal thickness on postoperative day 7. This novel model of pericardial patch angioplasty can be used to examine neointimal hyperplasia on vascular biomaterials, as well as to compare the differences between the arterial and venous environments.
心包补片常用于血管外科手术中闭合血管。为便于研究补片上形成的新生内膜增生，我们开发了一种补片血管成形术大鼠模型，该模型可用于静脉或动脉，分别创建补片静脉成形术或补片动脉成形术。本文讨论了该模型的技术细节。解剖肾下下腔静脉或主动脉，然后在近端和远端进行钳夹。在6至8周龄的Wistar大鼠的肾下下腔静脉或主动脉上进行3毫米的静脉切开术或动脉切开术。然后使用牛心包补片（3毫米×1.5毫米×0.6毫米），用10-0尼龙缝线闭合该部位。与动脉补片相比，静脉补片在术后第7天显示出新生内膜厚度增加。这种新型的心包补片血管成形术模型可用于研究血管生物材料上的新生内膜增生，以及比较动脉和静脉环境之间的差异。
