Poinsot Pierre, Penhoat Armelle, Mitchell Mélanie, Sauvinet Valérie, Meiller Laure, Louche-Pélissier Corinne, Meugnier Emmanuelle, Ruiz Mathias, Schwarzer Martin, Michalski Marie-Caroline, Leulier François, Peretti Noël
Institut de Génomique Fonctionnelle de Lyon, Université de Lyon, Ecole Normale Supérieure de Lyon, CNRS UMR5242, UCBL1, Lyon, France.
Univ Lyon, CarMeN Laboratory, INSERM, INRA, INSA Lyon, Université Claude Bernard Lyon 1, 69310, Pierre-Benite, France.
Pediatr Res. 2020 Sep;88(3):374-381. doi: 10.1038/s41390-020-0774-0. Epub 2020 Feb 5.
Chronic undernutrition leads to growth hormone resistance and poor growth in children, which has been shown to be modulated by microbiota. We studied whether Lactobacillus fermentum CECT5716 (Lf ), isolated from mother's breast milk, could promote juvenile growth through the modulation of lipid absorption in a model of starvation.
Germ-free (GF) Drosophila melanogaster larvae were inoculated with Lf in conditions of undernutrition with and without infant formula. The impact of Lf on larval growth was assessed 7 days after egg laying (AED) by measuring the larval size and on maturation by measuring the emergence of pupae during 21 days AED. For lipid absorption test, Caco2/TC7 intestinal cells were incubated with Lf and challenged with mixed lipid micelles.
The mono-associated larvae with Lf were significantly longer than GF larvae (3.7 vs 2.5 mm; p < 0.0001). The effect was maintained when Lf was added to the infant formula. The maturation time of larvae was accelerated by Lf (12 vs 13.2 days; p = 0.01). Lf did not have significant impact on lipid absorption in Caco2/TC7 cells.
Lf is a growth-promoting strain upon undernutrition in Drosophila, with a maintained effect when added to an infant formula but without effect on lipid absorption in vitro.
慢性营养不良会导致儿童生长激素抵抗和生长发育不良，研究表明微生物群可对此进行调节。我们研究了从母乳中分离出的发酵乳杆菌CECT5716（Lf）是否能在饥饿模型中通过调节脂质吸收来促进幼体生长。
在有无婴儿配方奶粉的营养不良条件下，给无菌（GF）黑腹果蝇幼虫接种Lf。在产卵后7天（AED）通过测量幼虫大小评估Lf对幼虫生长的影响，并在AED 21天内通过测量蛹的羽化情况评估其对成熟的影响。对于脂质吸收试验，将Caco2/TC7肠细胞与Lf一起孵育，并用混合脂质微团进行刺激。
与Lf单联的幼虫明显长于GF幼虫（3.7对2.5毫米；p<0.0001）。当将Lf添加到婴儿配方奶粉中时，这种效果得以维持。Lf加速了幼虫的成熟时间（12对13.2天；p=0.01）。Lf对Caco2/TC7细胞中的脂质吸收没有显著影响。
Lf是一种在果蝇营养不良时具有促生长作用的菌株，添加到婴儿配方奶粉中时效果得以维持，但对体外脂质吸收没有影响。
