Gavrilova Elizaveta, Kostenko Victoria, Zadorina Iva, Khusnutdinova Dilyara, Yarullina Dina, Ezhkova Asya, Bogachev Mikhail, Kayumov Airat, Nikitina Elena
Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia.
Department of Physiology and Patophysiology, Kazan State Academy of Veterinary Medicine Named after N.E. Bauman, 420029 Kazan, Russia.
Microorganisms. 2023 May 16;11(5):1297. doi: 10.3390/microorganisms11051297.
Probiotic bacteria exhibiting antagonistic activities against pathogenic bacteria are widely considered as potential options for the prevention and treatment of various infectious diseases and represent potential substitutes of antibiotics. Here we show that the AG10 strain represses the growth of and in vitro and diminishes their negative effects in vivo in a model of survival on embryonic (larvae) and pupa stages. In an agar drop diffusion test, AG10 exhibited antagonistic properties against , , and , and repressed the growth of and during milk fermentation. In a model, AG10 alone did not provide any significant effect, either during the embryonic stage or during further development of the flies. Despite this, it was able to restore the viability of groups infected with either and , almost to the level of non-treated control at all stages of development (larvae, pupa and adult). Moreover, in the presence of AG10, pathogens-induced mutation rates and recombination events reduced 1.5-2-fold. The genome of AG10 was sequenced and deposited at NCBI under the accession number PRJNA953814 and consists of annotated genome and raw sequence data. It consists of 109 contigs and is 3,479,919 bp in length with a GC content of 44.5%. The analysis of the genome has revealed considerably few putative virulence factors and three genes responsible for the biosynthesis of putative antimicrobial peptides, with one of them exhibiting a high probability of antimicrobial properties. Taken together, these data allow the suggestion that the AG10 strain is promising for use in both dairy production and probiotics as a preservative from foodborne infections.
对病原菌具有拮抗活性的益生菌被广泛认为是预防和治疗各种传染病的潜在选择，并且是抗生素的潜在替代品。在此我们表明，AG10菌株在体外抑制了[病原菌名称1]和[病原菌名称2]的生长，并在胚胎（幼虫）和蛹期存活模型中减轻了它们在体内的负面影响。在琼脂扩散试验中，AG10对[病原菌名称3]、[病原菌名称4]、[病原菌名称5]和[病原菌名称6]表现出拮抗特性，并在牛奶发酵过程中抑制了[病原菌名称7]和[病原菌名称8]的生长。在果蝇模型中，单独的AG10在胚胎期或果蝇进一步发育过程中均未产生任何显著影响。尽管如此，它能够在发育的所有阶段（幼虫、蛹和成虫）将感染[病原菌名称1]和[病原菌名称2]的组的活力恢复到几乎未处理对照的水平。此外，在AG10存在的情况下，病原体诱导的突变率和重组事件降低了1.5至2倍。AG10的基因组已测序并保存在NCBI，登录号为PRJNA953814，由注释基因组和原始序列数据组成。它由109个重叠群组成，长度为3,479,919 bp，GC含量为44.5%。基因组分析显示推定的毒力因子相当少，有三个负责推定抗菌肽生物合成的基因，其中一个具有很高的抗菌特性可能性。综上所述，这些数据表明AG10菌株有望用于乳制品生产和益生菌，作为预防食源性感染的防腐剂。
