Das Basanta Kumar, Ganguly Satabdi, Adhikari Anupam, Raut Subhasree Subhasmita, Pradhan Smruti Priyambada, Nag Subir Kumar, Kumari Kavita, Kumar Vikash
ICAR-Central Inland Fisheries Research Institute, Barrackpore, Kolkata, 700120, West Bengal, India.
Sci Rep. 2025 Mar 19;15(1):9479. doi: 10.1038/s41598-025-86513-x.
Cypermethrin (CYP), a persistent synthetic pyrethroid, poses a significant threat to aquatic life. The present work aims to identify the effect of CYP on the brain and liver transcriptome profile of fish Labeo catla. CYP (0.7 µg/L) was long-term exposed to L catla, and differentially expressed genes (DEGs) were investigated using RNA-sequencing (Illumina HiSeq2500). A total of 2665 and 18,020 unigenes and 333 and 454 DEGs were identified in the brain and liver transcriptome, respectively. DEGs associated with MAPK signaling and apoptosis pathways were co-expressed in both brain and liver transcriptome, according to pathway enrichment analysis. Concurrently, steroid and terpenoid backbone biosynthesis were overexpressed in liver, suggesting induction of apoptosis and steroid production, respectively. Among the identified DEGs, fosab, nr4a1, dhcr24 and tm7sf2 were significantly enriched and molecular docking studies further supported our findings. Long-term CYP exposure also altered the levels of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and malondialdehyde (MDA) content in L. catla. The present study provides significant insights into the consequences of CYP-induced toxicity in fish and identified functional genes that could be screened for estimating the toxic load of CYP in future ecotoxicological risk assessment studies.
氯氰菊酯（CYP）是一种持久性合成拟除虫菊酯，对水生生物构成重大威胁。本研究旨在确定CYP对印度野鲮鱼大脑和肝脏转录组图谱的影响。将CYP（0.7µg/L）长期暴露于印度野鲮鱼，使用RNA测序（Illumina HiSeq2500）研究差异表达基因（DEG）。在大脑和肝脏转录组中分别鉴定出总共2665个和18020个单基因以及333个和454个DEG。根据通路富集分析，与MAPK信号传导和凋亡通路相关的DEG在大脑和肝脏转录组中均共表达。同时，类固醇和萜类骨架生物合成在肝脏中过表达，分别表明诱导凋亡和类固醇产生。在鉴定出的DEG中，fosab、nr4a1、dhcr24和tm7sf2显著富集，分子对接研究进一步支持了我们的发现。长期暴露于CYP还改变了印度野鲮鱼中抗氧化酶超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）的水平以及丙二醛（MDA）含量。本研究为CYP诱导的鱼类毒性后果提供了重要见解，并鉴定出了功能性基因，这些基因可用于未来生态毒理学风险评估研究中估计CYP的毒性负荷筛选。
