Aiello Domenico, Ferradini Nicoletta, Torelli Lorenzo, Volpi Chiara, Lambalk Joep, Russi Luigi, Albertini Emidio
Department of Agricultural, Food and Environmental Sciences, University of Perugia, 06121 Perugia, Italy.
Enza Zaden Italia Research S.r.l. SS., 01016 Tarquinia, Italy.
Plants (Basel). 2020 Feb 1;9(2):175. doi: 10.3390/plants9020175.
Fennel () is a species belonging to the Apiaceae family, well known for its nutritional and pharmacological properties. Despite the economic and agricultural relevance, its genomic and transcriptomic data remain poor. Microsatellites-also known as simple sequence repeats (SSRs)-are codominant markers widely used to perform cross-amplification tests starting from markers developed in related species. SSRs represent a powerful tool, especially for those species lacking genomic information. In this study, a set of primers previously designed in for polymorphic SSR loci was tested in commercial varieties and breeding lines of fennel in order to: (i) test their cross-genera transferability, (ii) look at their efficiency in assessing genetic diversity, and (iii) identify their usefulness for marker-assisted selection (MAS) in breeding programs. Thirty-nine SSR markers from carrot were selected and tested for their transferability score, and only 23% of them resulted suitable for fennel. The low rate of SSR transferability between the two species evidences the difficulties of the use of genomic SSR in cross-genera transferability.
茴香（）是伞形科的一个物种，以其营养和药理特性而闻名。尽管具有经济和农业相关性，但其基因组和转录组数据仍然匮乏。微卫星——也称为简单序列重复（SSRs）——是共显性标记，广泛用于从相关物种中开发的标记开始进行交叉扩增测试。SSRs是一种强大的工具，尤其适用于那些缺乏基因组信息的物种。在本研究中，一组先前为多态性SSR位点设计的引物在茴香的商业品种和育种系中进行了测试，目的是：（i）测试它们的跨属转移性，（ii）观察它们在评估遗传多样性方面的效率，以及（iii）确定它们在育种计划中用于标记辅助选择（MAS）的实用性。从胡萝卜中选择了39个SSR标记并测试了它们的转移能力得分，其中只有23%适用于茴香。两个物种之间SSR转移率较低，这证明了在跨属转移性中使用基因组SSR的困难。
