DISAFA, Plant Genetics and Breeding, University of Turin, Largo P. Braccini 2, 10095 Grugliasco, Italy.
Biancheri Creazioni, 18033 Camporosso, Italy.
Int J Mol Sci. 2022 Mar 14;23(6):3126. doi: 10.3390/ijms23063126.
L. (2n = 2x = 16) is a perennial, allogamous, highly heterozygous plant marketed as a cut flower or in gardens. Due to its large genome size, limited efforts have been made in order to develop species-specific molecular markers. We obtained the first draft genome of the species by Illumina sequencing an androgenetic haploid plant of the commercial line "MISTRAL Magenta". The genome assembly was obtained by applying the MEGAHIT pipeline and consisted of 2 × 10 scaffolds. The SciRoKo SSR (Simple Sequence Repeats)-search module identified 401.822 perfect and 188.987 imperfect microsatellites motifs. Following, we developed a user-friendly " Microsatellite DataBase" (), which incorporates the Primer3 script, making it possible to design couples of primers for downstream application of the identified SSR markers. Eight genotypes belonging to eight cultivars were used to validate 62 SSRs and a subset of markers was applied for fingerprinting each cultivar, as well as to assess their intra-cultivar variability. The newly developed microsatellite markers will find application in Breeding Rights disputes, developing genetic maps, marker assisted breeding (MAS) strategies, as well as phylogenetic studies.
L.（2n = 2x = 16）是一种多年生、异花授粉、高度杂合的植物，作为切花或在花园中销售。由于其基因组较大，因此开发物种特异性分子标记的工作有限。我们通过对商业品系“MISTRAL Magenta”的雄核单倍体植物进行 Illumina 测序，获得了该物种的第一个草图基因组。基因组组装是通过应用 MEGAHIT 管道获得的，由 2×10 个支架组成。SciRoKo SSR（简单重复序列）搜索模块鉴定了 401822 个完美和 188987 个不完美的微卫星基序。随后，我们开发了一个用户友好的“微卫星数据库”（），其中包含 Primer3 脚本，可用于设计下游应用鉴定的 SSR 标记的引物对。使用 8 个属于 8 个品种的基因型来验证 62 个 SSR，选择一部分标记用于对每个品种进行指纹分析，并评估其品种内的变异性。新开发的微卫星标记将应用于品种权纠纷、遗传图谱的开发、标记辅助选择（MAS）策略以及系统发育研究。
