Chen Bolu, Hong Wuding, Yang Pengfei, Tang Yizhou, Zhao Yu, Aguilar Zoraida P, Xu Hengyi
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Zystein, LLC., Fayetteville, AR 72703, USA.
Nanomaterials (Basel). 2020 Feb 2;10(2):259. doi: 10.3390/nano10020259.
ZnO NPs have been assessed to show adverse effects on reproductive organs, but the molecular mechanisms of reproductive toxicity have not been sufficiently studied. In this research, the dosage effects from the oral exposure of ZnO NPs (30 nm) to pregnant mice in gestation day 10.5 to 17.5 was analyzed. Pregnant mice exposed to ZnO NPs induced dam injury, mice fetal growth restriction, and the fetus number decreased. The pathological evaluation showed that ZnO NPs exposure caused placental spongiotrophoblast area decease and structural damage. The RT-qPCR and immunocytochemistry data indicated that ZnO NPs could induce placenta oxide stress, endoplasmic reticulum stress responses, apoptosis, and altered placental function. These findings indicated that ZnO NPs could induce dam injury and fetal growth restriction. Reproductive toxicity of ZnO NPs may be due to placental injury and function alteration caused by apoptosis, oxide stress, and endoplasmic reticulum stress after ZnO NPs exposure.
已评估氧化锌纳米颗粒对生殖器官有不良影响，但生殖毒性的分子机制尚未得到充分研究。在本研究中，分析了妊娠第10.5至17.5天口服30纳米氧化锌纳米颗粒对怀孕小鼠的剂量效应。暴露于氧化锌纳米颗粒的怀孕小鼠出现母鼠损伤、小鼠胎儿生长受限且胎儿数量减少。病理评估表明，暴露于氧化锌纳米颗粒会导致胎盘海绵滋养层面积减少和结构损伤。实时定量聚合酶链反应和免疫细胞化学数据表明，氧化锌纳米颗粒可诱导胎盘氧化应激、内质网应激反应、细胞凋亡并改变胎盘功能。这些发现表明，氧化锌纳米颗粒可诱导母鼠损伤和胎儿生长受限。氧化锌纳米颗粒的生殖毒性可能是由于暴露后凋亡、氧化应激和内质网应激导致的胎盘损伤和功能改变。
