Yang Pengfei, Hong Wuding, Zhou Ping, Chen Bolu, Xu Hengyi
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Biochem Biophys Res Commun. 2017 Nov 25;493(3):1364-1369. doi: 10.1016/j.bbrc.2017.09.165. Epub 2017 Sep 30.
The oral ingestion of ZnO nanoparticles (NPs) has attracted considerable attention because of the wide usage in food packaging and additives. The small intestine is the major absorption site for ZnO NPs. Unfortunately, studies on the absorption of ZnO NPs in the GIT were still scarce. This study evaluated the absorption characteristics of ZnO NPs (30 nm) and bulk ZnO (rod morphology with a mean size of 139-846 nm). Results showed that ZnO NPs and bulk ZnO were absorbed and redistributed in various organs of mice at 4 h after exposure. Significantly higher levels of MT1 were observed in the duodenums of bulk ZnO and ZnO NPs groups than those of the control (9.8 and 5660.11 fold increases, respectively). The MT4 levels in the bulk ZnO and ZnO NPs groups also showed 4.07 and 43.21fold increases, respectively. In addition, the transcript levels of ZIPs, ZnTs, and MTs in the jejunum of bulk ZnO group were higher than those of ZnO NPs group. And the transcript levels of ZIPs, ZnTs, and MTs were all lower in the ileum than in the jejunum. The results suggested that ZnO NPs were mainly absorbed in the duodenum in the form of particles and can be absorbed as Zn in the jejunum and secondly in the ileum. By contrast, bulk ZnO was more easily absorbed as Zn in the jejunum and secondly in the ileum.
由于在食品包装和添加剂中的广泛应用，口服摄入氧化锌纳米颗粒（NPs）已引起了相当大的关注。小肠是氧化锌纳米颗粒的主要吸收部位。不幸的是，关于胃肠道中氧化锌纳米颗粒吸收的研究仍然很少。本研究评估了氧化锌纳米颗粒（30nm）和块状氧化锌（棒状形态，平均尺寸为139-846nm）的吸收特性。结果表明，暴露4小时后，氧化锌纳米颗粒和块状氧化锌在小鼠的各个器官中被吸收并重新分布。在块状氧化锌组和氧化锌纳米颗粒组的十二指肠中观察到的MT1水平显著高于对照组（分别增加了9.8倍和5660.11倍）。块状氧化锌组和氧化锌纳米颗粒组中的MT4水平也分别显示出4.07倍和43.21倍的增加。此外，块状氧化锌组空肠中ZIPs、ZnTs和MTs的转录水平高于氧化锌纳米颗粒组。回肠中ZIPs、ZnTs和MTs的转录水平均低于空肠。结果表明，氧化锌纳米颗粒主要以颗粒形式在十二指肠中被吸收，并可在空肠中以锌的形式被吸收，其次是在回肠中。相比之下，块状氧化锌更容易在空肠中以锌的形式被吸收，其次是在回肠中。
