Pan Lei, Duan Yin, Ma Feixia, Lou Lihua
The First School of Medicine, Zhejiang Chinese Medical University, Hangzhou, China.
The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China.
J Recept Signal Transduct Res. 2020 Apr;40(2):173-180. doi: 10.1080/10799893.2020.1719152. Epub 2020 Feb 6.
Breast cancer (BC) is one of the most common malignancies worldwide. Punicalagin (PN), which is a type of polyphenol, has been reported to act as a tumor suppressor. This study aimed to investigate the effects of PN on cellular process in BC and its molecular mechanism. The effects of various doses of PN on cell viability, migration, and invasion capacities of MCF-7 and MDA-MB-231 cells were detected by CCK-8, wound-healing, and Transwell assays. Golgi phosphoprotein 3 (GOLPH3) was then transfected into the cells with or without PN treatment, and GPLPH3 expression level was examined by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot, and expressions of epithelial-mesenchymal transition (EMT)-related protein matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9), E-Cadherin, and N-Cadherin were measured by Western blot. High dose of PN treatment (50 μM or higher) significantly inhibited viability, migration, and invasion of MCF-7 and MDA-MB-231 cells, while overexpressed GOLPH3 promoted cell viability, migration, and invasion, and partially reversed the effects of PN treatment on the BC cells. PN inhibited the expressions of GOLPH3, MMP-2, MMP-9, and N-Cadherin, and promoted E-Cadherin expression, while overexpression of GOLPH3 partly reversed above effects attributing to PN. Thus, PN suppresses cell viability and metastasis regulating GOLPH3 in BC, which provides a possible therapeutic direction to the treatment of BC.
乳腺癌（BC）是全球最常见的恶性肿瘤之一。石榴皮素（PN）是一种多酚，据报道具有肿瘤抑制作用。本研究旨在探讨PN对BC细胞过程的影响及其分子机制。通过CCK-8、伤口愈合和Transwell实验检测不同剂量PN对MCF-7和MDA-MB-231细胞活力、迁移和侵袭能力的影响。然后在有或无PN处理的情况下将高尔基体磷蛋白3（GOLPH3）转染到细胞中，通过定量实时聚合酶链反应（qRT-PCR）和蛋白质免疫印迹法检测GPLPH3表达水平，并通过蛋白质免疫印迹法检测上皮-间质转化（EMT）相关蛋白基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶-9（MMP-9）、E-钙黏蛋白和N-钙黏蛋白的表达。高剂量PN处理（50μM或更高）显著抑制MCF-7和MDA-MB-231细胞的活力、迁移和侵袭，而过表达GOLPH3促进细胞活力、迁移和侵袭，并部分逆转PN处理对BC细胞的影响。PN抑制GOLPH3、MMP-2、MMP-9和N-钙黏蛋白的表达，并促进E-钙黏蛋白表达，而GOLPH3过表达部分逆转了PN的上述作用。因此，PN通过调节BC中的GOLPH3抑制细胞活力和转移，这为BC的治疗提供了一个可能的治疗方向。
