鞣花酸和鞣花单宁对人成纤维细胞的体外影响：其治疗潜力的初步评价。
Effect of Punicalagin and Ellagic Acid on Human Fibroblasts In Vitro: A Preliminary Evaluation of Their Therapeutic Potential.
机构信息
Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Avda. Ilustración 60, 18016 Granada, Spain.
Institute of Biosanitary Research, Ibs.Granada, C/Doctor Azpitarte 4, 18012 Granada, Spain.
出版信息
Nutrients. 2023 Dec 20;16(1):23. doi: 10.3390/nu16010023.
BACKGROUND
Pomegranate is a fruit that contains various phenolic compounds, including punicalagin and ellagic acid, which have been attributed to anti-inflammatory, antioxidant, and anticarcinogenic properties, among others.
OBJECTIVE
To evaluate the effect of punicalagin and ellagic acid on the viability, migration, cell cycle, and antigenic profile of cultured human fibroblasts (CCD-1064Sk). MTT spectrophotometry was carried out to determine cell viability, cell culture inserts were used for migration trials, and flow cytometry was performed for antigenic profile and cell cycle analyses. Cells were treated with each phenolic compound for 24 h at doses of 10 to 10 M.
RESULTS
Cell viability was always significantly higher in treated versus control cells except for punicalagin at 10 M. Doses of punicalagin and ellagic acid in subsequent assays were 10 M or 10 M, which increased the cell migration capacity and upregulated fibronectin and α-actin expression without altering the cell cycle.
CONCLUSIONS
These in vitro findings indicate that punicalagin and ellagic acid promote fibroblast functions that are involved in epithelial tissue healing.
背景
石榴是一种水果，含有各种酚类化合物，包括安石榴甙和鞣花酸，具有抗炎、抗氧化和抗癌等特性。
目的
评估安石榴甙和鞣花酸对培养的人成纤维细胞（CCD-1064Sk）活力、迁移、细胞周期和抗原表型的影响。采用 MTT 分光光度法测定细胞活力，细胞培养小室用于迁移试验，流式细胞术用于抗原表型和细胞周期分析。用各酚类化合物在 10 到 10 M 的剂量处理细胞 24 小时。
结果
除了 10 M 的安石榴甙外，处理组细胞的活力总是显著高于对照组细胞。后续试验中安石榴甙和鞣花酸的剂量为 10 M 或 10 M，增加了细胞迁移能力，并上调了纤连蛋白和α-肌动蛋白的表达，而不改变细胞周期。
结论
这些体外研究结果表明，安石榴甙和鞣花酸促进了参与上皮组织愈合的成纤维细胞功能。
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